叶绿素荧光技术在植物抗逆性研究中具有良好的应用前景。以电导法测定结果为参照,研究适合鉴定茶树抗寒性的叶绿素荧光参数。结果表明,叶绿素荧光参数Fv/Fm配合Logistic方程测定的茶树品种抗寒能力与电导法的结果相一致：舒茶早>皖茶91>平阳特早茶,而Fv/Fo、Fm、Fo测定的结果则与之不相符。叶绿素荧光法在植物抗逆性鉴定过程中比电导法方便快捷,干扰因素少,可作为鉴定茶树品种抗寒性的一种新方法。
Application of chlorophyll fluorescence technique has shown a favorable prospect in the research field for plant resistance. In the present research, referring to the measurement results by electrolyte conductivity method, appropriate chlorophyll fluorescence parameters were studied for the determination of cold resistance of tea plants. The results showed that the cold resistance of different tea cultivars measured by chlorophyll fluorescence parameter Fv/Fm with logistic equation was the same as which were obtained by electrolyte conductivity method: cv.Shuchazao> cv.Wancha91> cv.Pingyangtezao. However, the determination conclusions by Fv/Fo, Fm and Fo were not correspond to the result acquired by electrolyte conductivity method. For the determination of plant resistance, we concluded that chlorophyll fluorescence method is more convenient and less interfered than the traditional electrolyte conductivity method. Therefore, it was treated as a new method to identify the cold resistance of tea plants.
[1] 骆颖颖, 梁月荣. 茶树抗寒性的间接鉴定[J]. 茶叶科学技术, 1998(3): 1-6.
[2] 朱根海, 刘祖祺, 朱培仁. 应用Logistic方程确定植物组织低温半致死温度的研究[J]. 南京农业大学学报, 1986(3): 11-16.
[3] 杨华, 唐茜, 黄毅, 等. 用电导法配合Logistic方程鉴定茶树的抗寒性[J]. 福建茶业, 2006(3): 30-32.
[4] 黄海涛, 余继忠, 张伟, 等. 电导法配合Logistic方程鉴定茶树抗寒性的探讨[J]. 浙江农业科学, 2009(3): 577-578.
[5] Smillie R M, Hetherington S E.Stress tolerance and stressinduced injury in crop plants measured by chlorophyll fluorescence in vivo: chilling, freezing, ice cover, heat and high light[J]. Plant Physiology, 1983, 72(4): 1043-1050.
[6] 周蕴薇, 刘艳萍, 戴思兰. 用叶绿素荧光分析技术鉴定植物抗寒性的剖析[J]. 植物生理学通讯, 2006, 42(5): 945-950.
[7] Johannes M A M CLEMENT, Philip R VAN HASSELT. Chlorophyll fluorescence as a parameter for frost hardiness in winter wheat[J]. Phyton,1996, 36(1): 29-41.
[8] Ying J, Lee EA, Tonenaar M.Response of maize leaf photosynthesis to low temperature during the grain-filling period[J]. Field Crops Res, 2000, 68(2): 87-96.
[9] Rose R, Haase D.Chlorophyll fluorescence and Variations in tissue cold hardiness in response to freezing stress in Douglas-fir seedlings[J]. New Forests, 2002, 23(2): 81-96.
[10] 王爱芳, 张钢, 魏士春, 等. 温度与光周期对樟子松实生苗针叶抗寒性的影响[J]. 中国农学通报, 2007, 23(2): 156-161.
[11] 惠红霞, 许兴, 李前荣. 外源甜菜碱对盐胁迫下枸杞光合功能的改善[J]. 西北植物学报, 2003, 23(12): 2137-2142.
[12] 赵丽英, 邓西平, 山仑. 不同水分处理下冬小麦旗叶叶绿素荧光参数的变化研究[J]. 中国生态农业学报, 2007, 15(1): 63-66.
[13] Perks MP, Osborne BA, Mitchell DT.Rapid predictions of cold tolerance in Douglas—fir seedlings using chlorophyll fluorescence after freezing[J]. New Forests, 2004, 28(1): 49-62.
[14] 李国景, 刘永华, 吴晓花, 等. 长豇豆品种耐低温弱光性和叶绿素荧光参数等的关系[J]. 浙江农业学报, 2005, 17(6): 359-362.
[15] 王可玢, 赵福洪, 王孝宣, 等. 用体内叶绿素a荧光动力学鉴定番茄的抗冷性[J]. 植物学通报,1996, 13(2): 29-33.
浙公网安备 33019902000101号 
