广东茶树种质资源AFLP分析

赵超艺; 周李华; 罗军武; 黄建安; 谭和平

doi:10.13305/j.cnki.jts.2006.04.003

茶叶科学 >

2006 , Vol. 26 >Issue 4: 249 - 252

DOI: https://doi.org/10.13305/j.cnki.jts.2006.04.003

广东茶树种质资源AFLP分析

赵超艺 ,
周李华 ,
罗军武 ,
黄建安 ,
谭和平

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1. 湖南农业大学园林园艺学院茶学系,湖南长沙 410128;
2. 教育部茶学重点实验室,湖南长沙 410128;
3. 广东省农业科学院茶叶研究所,广东英德 5103042;
4. 中国测试技术研究院,四川成都 610021

赵超艺（1962- ）,男,副研究员,主要研究方向茶树资源利用与研究

收稿日期: 2006-04-17

修回日期: 2006-07-17

网络出版日期: 2019-09-11

基金资助

广东省农科院资助项目(SR0273)

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AFLP Analysis of Genetic Diversity of Tea plant Germplasms in Guangdong Province

ZHAO Chao-yi ,
LUO Jun-wu ,
HUANG Jian-an ,
TAN He-ping

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1. Tea Science Department, Hunan Agricultural University, ChangSha 410128, China;
2. Key Laboratory of Tea Science of Ministry of Education, ChangSha 410128, China;
3. Tea Research Institute, GuangDong Academy of Agricultural Sciences, YingDe 513042, China;
4. National measure of institute and testing technology, ChengDu 610021, China

Received date: 2006-04-17

Revised date: 2006-07-17

Online published: 2019-09-11

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摘要

本研究采用AFLP技术,选用多态性高分辨能力强的5对引物对25个广东茶树群体品种和5个对照品种进行选择性扩增,获得了较清晰的DNA指纹图谱。5对引物共扩增出365条带,平均每对引物扩增73条带,多态性条带338条,占总带数的92.6%。扩增片段大小在50~600βbp之间。采用DPS 2000软件进行聚类,30份供试材料以清凉山茶和清远笔架茶遗传距离最近（0.12752）,桂北大叶种和凤凰水仙之间的遗传距离最远（0.5）,本实验结果表明广东地方茶树群体品种遗传多样性比较丰富。

关键词： 茶树; AFLP; 遗传分析

本文引用格式

赵超艺 , 周李华 , 罗军武 , 黄建安 , 谭和平 . 广东茶树种质资源AFLP分析[J]. 茶叶科学, 2006 , 26(4) : 249 -252 . DOI: 10.13305/j.cnki.jts.2006.04.003

Abstract

Based on AFLP method, AFLP figure were obtained in Guangdong tea plants. In this study, totally 365 bands were amplified by 5 pairs of primes and 73 bands were amplified by each primer pair on average, a total of 338 (92.6%) polymorphic AFLP markers were detected. The results indicated that the genetic distance (GD) based on AFLP data by using DPS 2000 was significant. The GD(0.5) is highest between Guibeidaye and Fenghuangshuixian, but the GD(0.12752) between Qingliangshan tea and Qingyuanbijia tea is low. This indicated that the Guangdong tea plant has a high genetic diversity.

Key words： tea plant[Camellia sinensis(L.)O.Kuntze]; AFLP; genetic diversity

参考文献

[1] Vos P.AFLP: a new technique for DNA fingerprint[J]. Nucl, Acids, Res, 1995(23): 4407~4414.
[2] M Labra, T Di Fabio, F Grassi, et al. AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants[J]. Chemosphere, 2003(52): 1183~1188.
[3] Katia Gil-Vega, Corina Dı´az, Alejandro Nava-Cedillo, ,et al. AFLP analysis of Agave tequilana varieties[J]. Plant Science. AFLP analysis of Agave tequilana varieties[J]. Plant Science, 2006(170): 904~909. [下转第258页]

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