探讨普洱茶对试验大鼠骨密度及骨钙、骨磷含量的影响。采用100只雄性Wistar大鼠按照体重随机分为对照组,普洱熟茶低、中、高剂量组,普洱生茶低、中、高剂量组和绿茶的低、中、高剂量组（共10组）,每组10只,分别按每千克体重0.5、1.0、2.0 g灌胃纯净水以及1 g/mL的普洱茶茶汤和绿茶茶汤,进行为期90 d的灌胃试验。试验结束后将实验大鼠处死,剥离左股骨,对骨钙骨磷含量、股骨密度等指标进行测定。结果显示,与对照组相比,高剂量普洱生茶、绿茶组及低、中剂量的普洱熟茶组骨钙含量显著高于对照组（P<0.05）;各试验组大鼠骨磷含量均显著高于对照组（P<0.05）;普洱茶(生茶和熟茶)和绿茶各剂量组与对照组相比骨密度均无显著性差异（P>0.05）。提示本试验条件下,本批次普洱茶对试验动物的骨密度没有影响。
The effects of Pu-erh tea on the indexes of bone mineral density and bone growth in wistar rats were investigated. 100 male wistar rats were divided into 10 groups of 10 rats each randomly. The first group severed as control. The 9 treatments groups were given daily oral administrations (20 mL/kg) of fermented Pu-erh tea, unfermented Pu-erh tea or green tea tract at the conversation listed above, which yielded dose of 0.5, 1.0 or 2.0 g/kg(body weight). The control group were given oral administrations of distilled water. After 90 d of treatment, the rats were sacrificed, peeled left femur and the weight of femur, the content of calcium and phosphorus in bone, the density of bones etc were determined. The resulted showed that compared to the control group, the weight of left femur in groups treated with either low, medium dose of unfermented Pu-erh tea and green tea or each dose of fermented Pu-erh tea was not statistically signification(P>0.05). The content of calcium in bone in groups treated with either the highest dose of unfermented Pu-erh tea and green tea or low, median dose of fermented Pu-erh tea was significantly higher than the control group(P<0.05) while the content of phosphorus in each group was significantly higher than the control group(P<0.05). The density of each group of fermented and unfermented Pu-erh tea and green tea was not statistically signification(P>0.05). Under the experimental conditions, these tea did not affect bone mineral density of rats.
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