本文在全自动智能化温室内,按组培+温室培育和直接温室扦插培育两条技术路线,采用不同材料进行了茶树工厂化育苗试验。结果表明,以田间材料为组培起始物时,春季新梢第三腋芽萌发率最好,每周期增殖率可达2.75倍左右,成苗率在20%以上,直接移植生根率为成活小苗的90%左右。一年双季育苗试验表明,春夏季育苗在3月中旬开始,5个月后,茶苗高度达20.4±8.3βcm,练苗后移栽成活率近100%;秋冬季育苗在8~9月初开始,冬季完成根系建立,开春后达20βcm以上高度,练苗后移栽;施肥处理茶苗高度的增加量是不施肥对照的5.9倍。
成浩
,
曾建明
,
周健
,
王丽鸳
,
常杰
,
葛滢
,
袁海波
,
谷保静
,
张小飞
. 茶树种苗工厂化快速繁育技术[J]. 茶叶科学, 2007
, 27(3)
: 231
-235
.
DOI: 10.13305/j.cnki.jts.2007.03.009
The rapid propagation of tea clonal seedling was carried out in auto-controlled greenhouse. The result showed that when the technical route of tissue culture-direct rooting-greenhouse incubating was followed, the optimal initial explant was the third axillary bud of spring shoots, which possessed the best germination rate, proliferated 2.75 times in one incubation cycle, and 20% of the cultures could grow to more than 5βcm length every cycles. On the other hand, when the technical route of direct greenhouse cutting and incubation was followed, biannual proliferation could be achieved with one started in middle March and harvested after 5 months while the height of seedlings reached 20.4±8.3βcm, the transplant survival rate of these seedlings was close to 100% after they were proper conditioned. The other proliferation started at about Aug. to Sept., after about 6 month’s incubation, the height of those seedlings reached more than 20βcm after the rootage was established during winter. The increment of seedling height of those fertilized was 5.9 times as much as those unfertilized.
[1] 闫杰, 罗庆熙, 韩丽萍. 工厂化育苗基质研究进展[J]. 中国蔬菜, 2006(2): 34~37.
[2] 赵兵. 瑞典的工厂化育苗及对我国的借鉴[J]. 中国林业, 2002(4B): 43.
[3] 袁华玲, 张金云, 张学义, 等. 蔬菜穴盘工厂化育苗技术及发展策略[J]. 安徽农业科学, 2003, 31(6): 977~979.
[4] 陈芳, 马显达, 陈娟. 北美红杉组培苗工厂化育苗技术[J]. 云南林业科技, 2003, 104(3): 11~13.
[5] 曾建明, 谷保静, 常杰, 等. 茶树工厂化育苗适宜基质水分条件研究[J]. 茶叶科学, 2005, 25(4): 270~274.
[6] 谷保静, 常杰, 曾建明, 等. 设施繁育茶苗适宜光照强度研究[J]. 茶叶科学, 2006, 26(1): 24~30.
[7] 周键, 成浩, 王丽鸳. 茶树组培快繁技术的优化研究[J]. 茶叶科学, 2005, 25(3): 172~176.
[8] 周键, 成浩, 王丽鸳. 激素处理对茶树组培苗温室内直接诱导生根的影响[J]. 茶叶科学, 2005, 25(4): 265~269.
[9] 成浩, 周健, 栾征, 等. CO2浓度对工厂化繁育茶苗光合和生长的影响[J]. 茶叶科学, 2007, 27(3): 226~230.
[10] 栾征, 曹前进, 成浩, 等. 冬季增温和延长光照对茶苗生长的影响[J]. 浙江大学学报(农业与生命科学版), 2007, 33(6) (印刷中).