选取提取时间、提取方式、提取溶剂以及提取溶剂用量这四个重要的提取参数,采用正交实验设计对茶叶中有机磷类农药残留的提取技术进行优化,减少了试验次数,提高了实验效率。应用SAS数据分析软件对实验数据进行分析,准确迅速得到了茶叶中有机磷类农药残留的提取最佳条件是用10βml乙酸乙酯作提取溶剂,震荡提取10 min。
The thesis optimized the extraction of organophosphorous(OPPS) in tea with orthogonal design of experiment. The specific volume of OPPS as variable was chosen and four important factors such as extraction time,extraction methods, solvent extraction, dosage of solvent extraction were selected. The experiment data was analyzed by statistical analysis system. Through the analysis, we got the best conditions of the extraction of OPPS in tea. The best condition of extraction of organophosphorous is 10 ml ethyl acetate with shake in 10 min for extraction.
[1] 王振川. 茶叶污染问题与控制技术途径[J]. 茶叶科学技术, 2007, 2: 33.
[2] 吴雪原, 盛旋. 三种农药在茶汤中的浸出对人体健康影响的风险评价[J]. 茶叶科学, 2007, 27(2): 141.
[3] 陈石榕. 对欧盟茶叶农药残留新标准的思考[J]. 农业标准化, 2000, 7: 28~29.
[4] 宛晓春, 蔡荟梅. 大力推进茶叶标准化,提高我国茶叶安全质量[J]. 茶业通报, 2008, 30(1): 6.
[5] 郑申西, 林华影. 有机氯农药残留量测定中几种前处理方法的比较与分析[J]. 中国公共卫生, 2001, 17(5): 448~449.
[6] 孙荣恒. 应用数理统计(第二版)[M]. 北京: 科学出版社, 2003.85-95
[7] 沈其君. SAS统计分析[M]. 南京:东南大学出版社, 2001. 100~105.
[8] SAS Institute Inc.SAS guide to macro processing (Version 6)(M). North Carolina: SAS Institute Inc, 1996.