通过添加十二烷基磺酸钠（SDS）为离子对试剂,以乙腈-水（磷酸）为流动相,在200 nm的检测波长条件下,建立了一种快速测定茶叶中茶氨酸的分析方法。茶氨酸的保留时间为6.27 min;在0.04~20 μg范围内,茶氨酸峰面积与进样量之间有良好的线性关系,回归方程为：Y=1.51466×106X+3.28706×105(r2=0.99901);当S/N=3时,最低检测浓度为1.15 ng。所建方法快速,简便,准确,可用于茶叶中茶氨酸含量测定。
A paired ion chromatographic method was developed for rapid determination of theanine in tea. SDS was added as a paired ion reagent, mobile phase was a mixture of acetonitrile and phosphoric acid aqueous solution, detection wavelength was set at 200 nm. The retention time of Theanine was 6.27 min. The calibration of theanine was in good linearity in the range of 0.04~20 μg with the regression equation Y=1.51466×106X+3.28706×105(r2=0.99901). The detection limits was 1.15 ng(S/N=3). This method is rapid, simple, and accurate for the analysis of theanine in tea.
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