采用四因素二次旋转正交回归组合设计方法对茶绿色素叶绿素锌钠盐的锌代工艺进行研究。结果表明：影响茶绿色素叶绿素锌钠盐得率最大的因素为锌代时间,其次为ZnSO4浓度、锌代温度、及锌代溶液pH值,其最佳制备工艺为溶液pH调至3.0,在72.8℃,用21.5%的ZnSO4溶液锌代89.6βmin,TGP-2产品得率可达0.69%。
：The manufacture processing of tea green pigment — sodium-zinc chlorophyllin (TGP-2) was studied by using expectant function approach for simultaneous optimization of several variable levels. The results showed that the effect of heating time was the most noticeable, then the concentration of ZnSO4,temperature, and pH successively. The optimum of preparation process was 3.0 pH, 72.8℃, with 21.5% of ZnSO4 concentration for 89.6βmin, and the efficiency of yield could reach 0.69%.
[1] 杨晓萍, 郭大勇, 张庆华. 新型茶绿色素叶绿素锌钠盐的研究[J]. 茶叶科学, 2003, 1(23): 27~30.
[2] 萧伟祥, 王勇, 李纯, 等. 茶绿色素的制取研究[J]. 安徽农学院学报, 1992, 19(3): 182~188.
[3] 王立娟, 李坚, 张丽君. 松针叶绿素锌钠的制备及稳定性[J]. 东北林业大学学报, 2004, 32(1): 50~52.
[4] 龚鹏飞, 曹伟峰, 冯淘. 竹叶叶绿素锌钠最佳制备工艺条件的确立[J]. 广州食品工业科技, 2004, 20(1): 64~67.
[5] 王巍杰, 孙彩云, 刘淑苹. 菠菜叶制取叶绿素锌钾盐及其稳定性[J]. 河北理工学院学报, 2005, 27(1): 125~128.
[6] Montgomery D C.Design and anylysis of experiments (3n edition)[M]. New York: John Wiley ＆ SOMS Inc, 1991
[7] 余家林. 农业多元试验统计[J]. 北京: 北京农业大学出版社. 1993
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