广东苦茶资源嘌呤生物碱含量分析与评价

李红建; 秦丹丹; 姜晓辉; 方开星; 王秋霜; 潘晨东; 李波; 王青; 吴华玲

doi:10.13305/j.cnki.jts.2021.01.008

茶叶科学 >

2021 , Vol. 41 >Issue 1: 71 - 79

DOI: https://doi.org/10.13305/j.cnki.jts.2021.01.008

研究报告

广东苦茶资源嘌呤生物碱含量分析与评价

李红建 ,
秦丹丹 ,
姜晓辉 ,
方开星 ,
王秋霜 ,
潘晨东 ,
李波 ,
王青 ,
吴华玲

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广东省农业科学院茶叶研究所/广东省茶树资源创新利用重点实验室,广东广州 510640

李红建,男,助理研究员,主要从事茶树资源与遗传育种研究,lihongjian.hi@163.com。

收稿日期: 2020-05-27

修回日期: 2020-06-24

网络出版日期: 2021-02-23

基金资助

广东省基础与应用基础研究基金（2020A1515110397）、国家现代农业产业技术体系建设专项（CARS-19）、广东省农业科学院院长基金（201830）、科技创新战略专项资金（高水平农科院建设）（R2017PY-QY009、R2018QD-99）

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Analysis and Evaluation of Purine Alkaloid Contents in Bitter Tea Germplasm Resources from Guangdong

LI Hongjian ,
QIN Dandan ,
JIANG Xiaohui ,
FANG Kaixing ,
WANG Qiushuang ,
PAN Chendong ,
LI Bo ,
WANG Qing ,
WU Hualing

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Tea Research institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Plant Resources Innovation and Utilization, Guangzhou 510640, China

Received date: 2020-05-27

Revised date: 2020-06-24

Online published: 2021-02-23

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摘要

以13份广东省境内收集的苦茶资源为研究对象,来自不同省份的28份代表性品种（系）为对照,分析苦茶资源中嘌呤生物碱含量特征。结果显示,咖啡碱、可可碱、苦茶碱及生物碱总量均表现出较大的遗传差异,遗传多样性指数（H′）为0.78~1.94,变异系数（CV）为34.89%~191.19%。系统聚类分析将试验材料分为6个类群,类群Ⅰ、Ⅱ、Ⅲ主要为常规品种（系）,咖啡碱是其优势嘌呤碱;类群Ⅴ为低咖啡碱的南昆山毛叶茶;类群Ⅳ、Ⅵ全部为苦茶资源,苦茶碱含量较高。通过综合评价,从苦茶资源中筛选出生物碱含量特异的育种材料10份。此外,不同叶位含量分析表明,苦茶中苦茶碱随叶片成熟度增加呈缓慢降低趋势,至成熟叶期仍维持较高水平。研究结果为今后嘌呤生物碱含量特异的功能性苦茶品种培育及开发利用提供了依据。

关键词： 聚类分析; 苦茶; 嘌呤生物碱; 遗传多样性; 种质资源

本文引用格式

李红建 , 秦丹丹 , 姜晓辉 , 方开星 , 王秋霜 , 潘晨东 , 李波 , 王青 , 吴华玲 . 广东苦茶资源嘌呤生物碱含量分析与评价[J]. 茶叶科学, 2021 , 41(1) : 71 -79 . DOI: 10.13305/j.cnki.jts.2021.01.008

Abstract

The purine alkaloids of 13 bitter tea germplasms from Guangdong Province along with 28 representative cultivars (lines) from different tea producing areas in China were selected for analysis in this paper. The results indicate that genetic diversity index (H′) of purine alkaloids ranged from 0.78 to 1.94, while the coefficient of variation (CV) ranged from 34.89% to 191.19%. The samples could be divided into 6 groups based on cluster analysis: group Ⅰ, Ⅱ and Ⅲ were predominantly consisted of conventional cultivars with high caffeine content; group Ⅴ contained caffeine-free cultivars from Nankun Mountain, while all samples in group Ⅳ and Ⅵ were bitter tea with high theacrine content. A total of 10 purine alkaloid specific bitter tea resources were identified. Moreover, theacrine showed a gradually decrease trend with the increase of leaf positions but maintained a high level in mature leaves of bitter tea. Taken together, our research provided a basis for genetic improvement of functional tea cultivars with specific purine alkaloids in the future.

Key words： bitter tea; cluster analysis; genetic diversity; germplasms; purine alkaloids

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