厌氧处理条件下紫娟茶化学成分的变化及其对体外ACE活性的影响

杨高中; 施江; 张悦; 彭群华; 林智; 吕海鹏

doi:10.13305/j.cnki.jts.2023.04.007

茶叶科学 >

2023 , Vol. 43 >Issue 4: 553 - 566

DOI: https://doi.org/10.13305/j.cnki.jts.2023.04.007

研究报告

厌氧处理条件下紫娟茶化学成分的变化及其对体外ACE活性的影响

杨高中 ,
施江 ,
张悦 ,
彭群华 ,
林智 ,
吕海鹏

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1.中国农业科学院茶叶研究所/农业农村部特种经济动植物生物学与遗传育种重点实验室,浙江杭州 310008;
2.中国农业科学院研究生院,北京 100081

杨高中,男,硕士研究生,主要从事茶叶品质化学与营养健康方面研究。

收稿日期: 2023-04-17

修回日期: 2023-05-14

网络出版日期: 2023-08-24

基金资助

国家茶叶产业技术体系（CARS-19）、中国农业科学院科技创新工程项目（CAAS-ASTIP-2014-TRICAAS）

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Changes in Chemical Composition of Zijuan Tea under Anaerobic Treatment Conditions and Their Effects on in vitro ACE Activity

YANG Gaozhong ,
SHI Jiang ,
ZHANG Yue ,
PENG Qunhua ,
LIN Zhi ,
LÜ Haipeng

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1. Tea Research Institute of the Chinese Academy of Agricultural Science/Key Laboratory of Biology, Genetics and Breeding of SpecialEconomic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China;
2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2023-04-17

Revised date: 2023-05-14

Online published: 2023-08-24

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摘要

以紫娟茶树鲜叶为研究对象,对厌氧处理后制备的冻干样、绿茶、红茶、白茶等茶样中主要非挥发性成分（包括儿茶素、氨基酸、花青素等）进行测定,并采用高效液相色谱法评估其体外血管紧张素转换酶（Angiotensin-converting enzyme,ACE）抑制活性;此外,尝试通过分子对接模拟试验研究茶叶中主要化学成分和ACE之间潜在的互作机制。结果表明,经厌氧处理后的冻干样中γ-氨基丁酸（γ-aminobutyric acid,GABA）含量增加至1.72 mg·g^-1,显著高于未厌氧处理茶样的0.04 mg·g^-1（P<0.05）。厌氧处理后不同的加工工艺对茶样中的氨基酸和多酚类化合物的组成及含量影响较大;采用白茶工艺能提高茶样中氨基酸组分含量,而冻干工艺能最大程度保留茶样中多酚类化合物含量。紫娟茶树鲜叶样品经厌氧处理后,体外ACE抑制活性显著增强（P<0.05）;厌氧处理后采用冷冻干燥工艺所制样品的ACE抑制活性最强,显著高于采用绿茶、白茶和红茶加工工艺制作的样品（P<0.05）。此外,分子对接模拟试验发现,茶叶中的主要化学成分与ACE的相互作用强度与其分子结构中的亲水基团和芳香环结构有关。研究结果有助于揭示厌氧处理对紫娟茶ACE抑制活性的影响,为开发具有潜在治疗高血压等特殊功能型紫娟茶产品提供理论依据。

关键词： γ-氨基丁酸; 茶; 加工工艺; 血管紧张素转换酶; 厌氧处理

本文引用格式

杨高中 , 施江 , 张悦 , 彭群华 , 林智 , 吕海鹏 . 厌氧处理条件下紫娟茶化学成分的变化及其对体外ACE活性的影响[J]. 茶叶科学, 2023 , 43(4) : 553 -566 . DOI: 10.13305/j.cnki.jts.2023.04.007

Abstract

In this study, fresh leaves of Zijuan tea were treated with anaerobic treatment and used to make freeze-dried, green, black and white tea samples. The main non-volatile components (such as catechins, amino acids, anthocyanins) in these samples were determined and their in vitro angiotensin-converting enzyme (ACE) inhibitory activities were evaluated by high performance liquid chromatography. In addition, molecular docking simulation experiments were conducted to investigate the potential interaction mechanisms between the major chemical components in tea and ACE. The results show that the GABA content in the freeze-dried samples after anaerobic treatment increased to 1.72 mg·g^-1, which was significantly higher than that in the tea samples without anaerobic treatment (0.04 mg·g^-1, P<0.05). The composition and contents of amino acids and polyphenolic compounds in the tea samples were significantly affected by the processing methods after anaerobic treatment. The white tea process could increase the contents of amino acid components in the tea samples, while the freeze-drying process could maximize the retention of polyphenolic compounds in the tea samples. The in vitro ACE inhibitory activity of Zijuan tea fresh leaf samples was significantly enhanced after anaerobic treatment (P<0.05); the strongest ACE activity inhibition was observed in samples prepared by freeze-drying process after anaerobic treatment, which was significantly higher than that of samples prepared by green, white, and black tea processing methods (P<0.05). In addition, molecular docking simulation experiments reveal that the strength of the interaction between the main chemical components in tea and ACE was related to the hydrophilic groups and aromatic ring structures in their molecular structures. The results of the study helped to reveal the effect of anaerobic treatment on the ACE inhibitory activity of Zijuan tea and provided a theoretical basis for the development of special functional Zijuan tea products with potential treatment of hypertension.

Key words： γ-aminobutyric acid; anaerobic treatment; angiotensin-converting enzyme; processing technology; tea

参考文献

[1] Zhou B, Carrillo-larco R M, Danaei G, et al. Worldwide trends in hypertension prevalence and progress in treatment and control from 1990 to 2019: a pooled analysis of 1201 population-representative studies with 104 million participants[J]. The Lancet, 2021, 398(10304): 957-980.
[2] Li X C, Zhang J, Zhuo J L.The vasoprotective axes of the renin-angiotensin system: physiological relevance and therapeutic implications in cardiovascular, hypertensive and kidney diseases[J]. Pharmacological Research, 2017, 125: 21-38.
[3] Wu J, Liao W, Udenigwe C C.Revisiting the mechanisms of ACE inhibitory peptides from food proteins[J]. Trends in Food Science & Technology, 2017, 69: 214-219.
[4] Yang G Z, Meng Q, Shi J, et al.Special tea products featuring functional components: health benefits and processing strategies[J]. Comprehensive Reviews in Food Science and Food Safety, 2023, 22(3): 1686-1721.
[5] Shi J, Simal-gandara J, Mei J, et al. Insight into the pigmented anthocyanins and the major potential co-pigmented flavonoids in purple-coloured leaf teas[J]. Food Chemistry, 2021, 363: 130278. doi: 10.1016/j.foodchem.2021.130278.
[6] Dong J, Xu X, Liang Y, et al.Inhibition of angiotensin converting enzyme (ACE) activity by polyphenols from tea (Camellia sinensis) and links to processing method[J]. Food & Function, 2011, 2(6): 310. doi: 10.1039/c1fo10023h.
[7] Kurita I, Maeda-yamamoto M, Tachibana H, et al. Antihypertensive effect of Benifuuki tea containing O-methylated EGCG[J]. Journal of Agricultural and Food Chemistry, 2010, 58(3): 1903-1908.
[8] Fan W D, Zong H R, Zhao T, et al.Bioactivities and mechanisms of dietary proanthocyanidins on blood pressure lowering: a critical review of in vivo and clinical studies[J]. Critical Reviews in Food Science and Nutrition, 2022: 1-17. doi: 10.1080/10408398.2022.2132375.
[9] Ockermann P, Headley L, Lizio R, et al.A review of the properties of anthocyanins and their influence on factors affecting cardiometabolic and cognitive health[J]. Nutrients, 2021, 13(8): 2831. doi: 10.3390/nu13082831.
[10] 包云秀, 夏丽飞, 李友勇, 等. 茶树新品种‘紫娟’[J]. 园艺学报, 2008, 35(6): 934.
Bao Y X, Xia L F, Li Y Y, et al.A new tea tree cultivar ‘Zjuan’[J]. Acta Horticulturae Sinica, 2008, 35(6): 934.
[11] Hinton T, Johnston G A R. GABA-enriched teas as neuro-nutraceuticals[J]. Neurochemistry International, 2020, 141: 104895. doi: 10.1016/j.neuint.2020.104895.
[12] Dai W D, Xie D C, Lin Z, et al.A nontargeted and targeted metabolomics study on the dynamic changes in metabolite levels during the anaerobic treatment of γ-aminobutyric acid (GABA) tea[J]. LWT, 2020, 126: 109313. doi: 10.1016/j.lwt.2020.109313.
[13] Chen Q, Zhang Y M, Tao M M, et al.Comparative metabolic responses and adaptive strategies of tea leaves (Camellia sinensis) to N₂ and CO₂ anaerobic treatment by a nontargeted metabolomics approach[J]. Journal of Agricultural and Food Chemistry, 2018, 66(36): 9565-9572.
[14] Yin Z T, Yan R Y, Jiang Y S, et al.Identification of peptides in Qingke baijiu and evaluation of its angiotensin converting enzyme (ACE) inhibitory activity and stability[J]. Food Chemistry, 2022, 395: 133551. doi: 10.1016/j.foodchem.2022.133551.
[15] Wei D, Fan W L, Xu Y.Identification of water-soluble peptides in distilled spent grain and its angiotensin converting enzyme (ACE) inhibitory activity based on UPLC-Q-TOF-MS and proteomics analysis[J]. Food Chemistry, 2021, 353: 129521. doi: 10.1016/j.foodchem.2021.129521.
[16] Yang C, Hu Z Y, Lu M L, et al.Application of metabolomics profiling in the analysis of metabolites and taste quality in different subtypes of white tea[J]. Food Research International, 2018, 106: 909-919.
[17] Da Silva T B V, Castilho P A, De Sá-nakanishi A B, et al. The inhibitory action of purple tea on in vivo starch digestion compared to other Camellia sinensis teas[J]. Food Research International, 2021, 150: 110781. doi: 10.1016/j.foodres.2021.110781.
[18] 杨高中, 彭群华, 张悦, 等. 厌氧处理对不同类型茶叶的氨基酸组成及生物活性的影响[J]. 茶叶科学, 2022, 42(2): 222-232.
Yang G Z, Peng Q H, Zhang Y, et al.Effects of anaerobic treatment on amino acid composition and biological activities of different type teas[J]. Journal of Tea Science, 2022, 42(2): 222-232.
[19] Wang Y, Kan Z, Thompson H J, et al.Impact of six typical processing methods on the chemical composition of tea leaves using a single Camellia sinensis cultivar, Longjing 43[J]. Journal of Agricultural and Food Chemistry, 2019, 67(19): 5423-5436.
[20] Yu F, Chen C, Chen S N, et al.Dynamic changes and mechanisms of organic acids during black tea manufacturing process[J]. Food Control, 2022, 132: 108535. doi: 10.1016/j.foodcont.2021.108535.
[21] 吕海鹏, 杨停, 梁名志, 等. “紫娟”茶中的EGCG3"Me成分研究[J]. 现代食品科技, 2014, 30(9): 286-289, 296.
Lü H P, Yang T, Liang M Z, et al.Study of EGCG3"Me content in Zijuan tea[J]. Modern Food Science and Technology, 2014, 30(9): 286-289, 296.
[22] 时鸿迪, 王邦政, 李乾, 等. 不同加工工艺下‘紫娟’茶品质的比较[J]. 中国农学通报, 2020, 36(34): 142-147.
Shi H D, Wang B Z, Li Q, et al.The quality of ‘Zijuan’ tea under different processing techniques: a comparative analysis[J]. Chinese Agricultural Science Bulletin, 2020, 36(34): 142-147.
[23] Lü H P, Dai W D, Tan J F, et al.Identification of the anthocyanins from the purple leaf coloured tea cultivar Zijuan (Camellia sinensis var. assamica) and characterization of their antioxidant activities[J]. Journal of Functional Foods, 2015, 17: 449-458.
[24] Tu J, Liu G H, Jin Y C, et al.Enrichment of γ-aminobutyric acid in mulberry leaves and the inhibitory effects of the water extract on ACE and α-glucosidase activity[J]. Industrial Crops and Products, 2022, 177: 114485. doi: 10.1016/j.indcrop.2021.114485.
[25] Wu Y, Han Z S, Wen M C, et al.Screening of α-glucosidase inhibitors in large-leaf yellow tea by offline bioassay coupled with liquid chromatography tandem mass spectrometry[J]. Food Science and Human Wellness, 2022, 11(3): 627-634.
[26] Diana M, Quílez J, Rafecas M.Gamma-aminobutyric acid as a bioactive compound in foods: a review[J]. Journal of Functional Foods, 2014, 10: 407-420.
[27] Liu Z, Bruins M E, Ni L, et al.Green and black tea phenolics: bioavailability, transformation by colonic microbiota, and modulation of colonic microbiota[J]. Journal of Agricultural and Food Chemistry, 2018, 66(32): 8469-8477.
[28] Wang X M, Chen H X, Fu X G, et al.A novel antioxidant and ACE inhibitory peptide from rice bran protein: biochemical characterization and molecular docking study[J]. LWT, 2017, 75: 93-99. doi: 10.1016/j.lwt.2016.08.047.
[29] Shih Y H, Chen F A, Wang L F, et al.Discovery and study of novel antihypertensive peptides derived from cassia obtusifoliaseeds[J]. Journal of Agricultural and Food Chemistry, 2019, 67(28): 7810-7820.
[30] Mirzaei M, Mirdamadi S, Ehsani M R, et al.Production of antioxidant and ACE-inhibitory peptides from Kluyveromyces marxianus protein hydrolysates: purification and molecular docking[J]. Journal of Food and Drug Analysis, 2018, 26(2): 696-705.
[31] Shukor N A, Van Camp J, Gonzales G B, et al.Angiotensin-converting enzyme inhibitory effects by plant phenolic compounds: a study of structure activity relationships[J]. Journal of Agricultural and Food Chemistry, 2013, 61(48): 11832-11839. doi:10.1021/jf404641v.

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