茶叶科学 >

2024 , Vol. 44 >Issue 2: 219 - 230

DOI: https://doi.org/10.13305/j.cnki.jts.2024.02.010

研究报告

氟处理下茶树根系的组织细胞形态差异及蜡质合成相关基因WSD1的表达分析

  • 宋博 ,
  • 贾培凝 ,
  • 叶文祺 ,
  • 吴俊 ,
  • 孙威江 ,
  • 薛志慧
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  • 1.福建农林大学园艺学院,福建 福州 350002;
    2.福建农林大学安溪茶学院,福建 安溪 362406;
    3.福建省茶产业工程技术研究中心,福建 福州 350002
宋博,女,硕士研究生,主要从事茶树分子生物学方面的研究。

收稿日期: 2023-08-22

  修回日期: 2023-11-26

  网络出版日期: 2024-04-30

基金资助

国家重点研发计划项目（2019YFD1001601）、福建农林大学“双一流”建设科技创新能力提升培育计划项目（KSYLC005）、福建张天福茶叶发展基金会科技创新基金（FJZTF01）

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Physiological Differences and Expression Analysis of Wax Synthesis Related Gene WSD1 in Tea Roots Treated with Fluorine

  • SONG Bo ,
  • JIA Peining ,
  • YE Wenqi ,
  • WU Jun ,
  • SUN Weijiang ,
  • XUE Zhihui
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  • 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Anxi College of Tea Science, Fujian Agriculture and Forestry University, Anxi 362406, China;
    3. Tea Industry Engineering Technology Research Center of Fujian Province, Fuzhou 350002, China

Received date: 2023-08-22

  Revised date: 2023-11-26

  Online published: 2024-04-30

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摘要

茶树是一种氟含量较高的植物,具有聚氟性。基于扫描电镜技术,探究不同质量浓度（10 mg·L-1和50 mg·L-1）及不同时间（1 d和16 d）氟处理下,氟高富集茶树品种黄棪和氟低富集茶树品种佛手根系吸收富集氟的特性差异,并对前期转录组数据筛选出氟处理下茶树蜡质合成的差异基因WSD1进行分析。结果显示,50 mg·L-1氟处理后,黄棪根部表皮细胞表面覆盖的蜡质稍有增多,细胞排列较为疏松,而佛手根部表皮细胞界限模糊,覆盖蜡质明显增多,细胞壁出现扭曲断裂等氟不耐受症状。蜡质合成相关基因WSD1荧光定量结果显示,WSD1经外源氟处理后,其对茶树根部的蜡质有较为明显的上调作用;WSD1蛋白互作网络预测结果及相关性分析显示,WSD1受CSS0041298、CSS0012327及CSS0049082的负调控。本研究从茶树与蜡质合成互作角度探究茶树缓解氟胁迫的响应机制,为深入探究茶树氟吸收调控和耐氟茶树品种的选育提供科学依据。

关键词： SEM; 茶树; 氟; 蜡质合成基因

本文引用格式

宋博 , 贾培凝 , 叶文祺 , 吴俊 , 孙威江 , 薛志慧 . 氟处理下茶树根系的组织细胞形态差异及蜡质合成相关基因WSD1的表达分析[J]. 茶叶科学, 2024 , 44(2) : 219 -230 . DOI: 10.13305/j.cnki.jts.2024.02.010

Abstract

Tea plant, as one of the plants with high fluorine contents, has fluoride-polymerizing property. In this paper, based on scanning electron microscopy, the roots of ‘Huangdan’ and ‘Foshou’ were treated with different fluoride concentrations (10 mg·L-1 and 50 mg·L-1) and different time periods (1 d and 16 d). The differentially expressed gene WSD1 of tea wax synthesis under fluoride treatment was screened from the transcriptome data of our research group. The results show that under 50 mg·L-1 fluoride treatment, the epidermal cells of ‘Huangdan’ root had slightly more wax on the surface and relatively loose cell arrangement, while the epidermal cells of ‘Foshou’ root had blurred boundaries, significantly more wax on the surface, and fluorine intolerance symptoms such as cell wall distortion and breakage. Quantitative fluorescence results of WSD1 related to wax synthesis show that WSD1 had a significant up-regulation effect on the wax content of tea root under exogenous fluoride treatment. The prediction results of WSD1 protein interaction network and correlation analysis show that WSD1 was negatively regulated by CSS0041298, CSS0012327 and CSS0049082. This study provided a theoretical reference for alleviating fluorine stress in tea plants from the perspective of the interaction between tea plants and wax synthesis, and provided a scientific basis for further exploring the regulation of fluorine absorption in tea plants and the breeding of fluorine-tolerant tea cultivars.

Key words： fluorine; SEM; tea plant; wax synthetic genes

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