广元黄茶自组装锌复合物的制备及其抗菌抗炎活性研究

安鲁敬; 钱益跃; 常可; 田宝明; 穆丹; 张明珠; 陈红平; 张相春

doi:10.13305/j.cnki.jts.2026.01.005

茶叶科学 >

2026 , Vol. 46 >Issue 1: 139 - 150

DOI: https://doi.org/10.13305/j.cnki.jts.2026.01.005

研究报告

广元黄茶自组装锌复合物的制备及其抗菌抗炎活性研究

安鲁敬 ,
钱益跃 ,
常可 ,
田宝明 ,
穆丹 ,
张明珠 ,
陈红平 ,
张相春

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1.皖西南生物多样性研究与生态保护安徽省重点实验室,安庆师范大学生命科学学院,安徽安庆 246133;
2.茶树种质创新与资源利用全国重点实验室,中国农业科学院茶叶研究所,浙江杭州 310008;
3.浙江省金华生态环境监测中心,浙江金华 321000

安鲁敬,男,硕士研究生,主要从事茶精深加工健康利用方面的研究。

收稿日期: 2025-10-15

修回日期: 2025-11-28

网络出版日期: 2026-02-06

基金资助

四川省重点研发计划（2024YFHZ0179）; 安徽省新时代育人质量工程项目（研究生教育）教学研究一般项目（2023jyjxggyjY194）; 安徽省自然科学基金青年项目（2308085QC78）

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Preparation of Guangyuan Yellow Tea Self-Assembled Zinc Nanocomposite and Their Antibacterial and Anti-Inflammatory Activities

AN Lujing ,
QIAN Yiyue ,
CHANG Ke ,
TIAN Baoming ,
MU Dan ,
ZHANG Mingzhu ,
CHEN Hongping ,
ZHANG Xiangchun

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1. The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, School of Life Sciences, Anqing Normal University, Anqing 246133, China;
2. State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
3. Zhejiang Jinhua Ecological and Environmental Monitoring Center, Jinhua 321000, China

Received date: 2025-10-15

Revised date: 2025-11-28

Online published: 2026-02-06

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摘要

目前关于直接以茶浸提液为原料构建新型功能复合物研究较少,本研究以广元黄茶茶汤和硫酸锌为原料,通过绿色简便的一步自组装法成功制备了广元黄茶-锌纳米复合物（Y-Zn）。通过紫外-可见分光光度计（UV-vis）、透射电子显微镜（TEM）、动态光散射（DLS）、傅里叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）表征显示,所合成的纳米复合物粒径均一、分散性良好。抗菌试验表明,Y-Zn对包括耐药菌株在内的多种革兰氏阳性菌表现出剂量依赖性的抗菌效果,并能有效抑制细菌生物膜形成;抗氧化试验结果显示,Y-Zn具备优异的自由基清除能力,在20 μg·mL^-1质量浓度下清除率超过80%。细胞试验证实,Y-Zn通过下调诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的表达,抑制脂多糖（LPS）诱导的巨噬细胞炎症反应,且斑马鱼模型显示其生物安全性良好。本研究直接以茶汤为对象开发出一种兼具多种生物活性和安全性的自组装复合物,为我国丰富茶叶资源在生命健康领域中的应用提供了思路。

关键词： 茶汤; 抗菌活性; 抗炎活性; 纳米复合物; 自组装

本文引用格式

安鲁敬 , 钱益跃 , 常可 , 田宝明 , 穆丹 , 张明珠 , 陈红平 , 张相春 . 广元黄茶自组装锌复合物的制备及其抗菌抗炎活性研究[J]. 茶叶科学, 2026 , 46(1) : 139 -150 . DOI: 10.13305/j.cnki.jts.2026.01.005

Abstract

Currently, few studies have reported on the direct use of tea extracts as raw materials for creating novel functional composites. This study successfully synthesized Guangyuan Yellow Tea-Zinc Nanocomposites (Y-Zn) using Guangyuan Yellow Tea infusion and zinc sulfate as raw materials through a green, simple one-step self-assembly method. Characterization via UV-vis spectrophotometry, transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) reveals that the synthesized nanocomposite exhibits uniform particle size and excellent dispersion. Antibacterial experiments demonstrated that Y-Zn exhibits dose-dependent antibacterial effects against multiple Gram-positive bacteria, including drug-resistant strains, and effectively inhibits bacterial biofilm formation. Antioxidant experiments revealed its excellent free radical scavenging ability, achieving over 80% scavenging efficiency at a concentration of 20 μg·mL^-1. Cellular experiments confirmed that Y-Zn suppresses lipopolysaccharide (LPS)-induced macrophage inflammatory responses by downregulating inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Furthermore, zebrafish models demonstrated its favorable biosafety profile. In summary, this study directly utilized tea infusion as the subject to develop a self-assembled composite that possesses multiple biological activities and safety, providing insights into the application of China's abundant tea resources in the field of life sciences and health.

Key words： antibacterial activity; anti-inflammatory activity; nanocomposite; self-assembly; tea infusion

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