西藏墨脱引起茶树叶枯病的两种镰刀菌新病原鉴定和生物学特性

金怀; 崔婷; 王楠; 魏丽萍; 张荣; 巩文峰

doi:10.13305/j.cnki.jts.2026.02.007

茶叶科学 >

2026 , Vol. 46 >Issue 2: 318 - 330

DOI: https://doi.org/10.13305/j.cnki.jts.2026.02.007

研究报告

西藏墨脱引起茶树叶枯病的两种镰刀菌新病原鉴定和生物学特性

金怀 ,
崔婷 ,
王楠 ,
魏丽萍 ,
张荣 ,
巩文峰

展开

1.西藏农牧大学植物科学学院,西藏林芝 860000;
2.西藏农牧大学林草学院,西藏林芝 860000;
3.农心作物科技股份有限公司,陕西西安 710065

金怀,男,硕士研究生,主要从事茶树真菌病害方面的研究,jinhuai.cx@outlook.com。

收稿日期: 2025-12-04

修回日期: 2026-01-10

网络出版日期: 2026-04-22

基金资助

西藏农牧学院研究生创新项目（YJS2024-04）; 西藏自治区重点研发计划（XZ202201ZY0011N）; 西藏农牧学院林学博士点（一期）（533325001）; 西藏自治区中央引导地方项目（XZ202201YD0038C）

收起

Identification and Biological Characterization of Two Novel Fusarium Pathogen Causing Tea Leaf Blight in Motuo Conty, Xizang

JIN Huai ,
CUI Ting ,
WANG Nan ,
WEI Liping ,
ZHANG Rong ,
GONG Wenfeng

Expand

1. Xizang Agricultural and Animal Husbandry University, College of Plant Science, Linzhi 860000, China;
2. Xizang Agricultural and Animal Husbandry University, College of Forestry and Grassland, Linzhi 860000, China;
3. Norsyn Crop Science and Technology Co., Ltd., Xi'an 710065, China

Received date: 2025-12-04

Revised date: 2026-01-10

Online published: 2026-04-22

Fold

摘要

2024年6—7月从西藏林芝墨脱县茶树叶枯病病叶中分离获得2种镰刀菌（Fusarium spp.）菌株,结合形态学特征、系统发育分析及科赫氏法则对其进行菌种鉴定。结果显示,2种镰刀菌分别被鉴定为Fusarium dracaenophilum和Fusarium asiaticum。科赫氏法则检验证实,F. dracaenophilum与F. asiaticum均能诱发茶树叶枯病。不同茶树品种致病性研究显示,‘中黄1号’对两种病原的抗病性显著强于‘福鼎大白茶’。生物学特性研究发现,F. asiaticum的适宜生长温度为20~25 ℃,pH为7~9,蔗糖、麦芽糖、乳糖和酵母粉有利于其生长;F. dracaenophilum最适生长温度为25 ℃,最适pH为7,乳糖和酵母粉有利于其生长。研究结果丰富了镰刀菌病原多样性认知,为西藏茶园叶部病害防控提供了理论依据。

关键词： 茶树叶枯病; 多基因系统发育分析; 镰刀菌; 形态学鉴定

本文引用格式

金怀 , 崔婷 , 王楠 , 魏丽萍 , 张荣 , 巩文峰 . 西藏墨脱引起茶树叶枯病的两种镰刀菌新病原鉴定和生物学特性[J]. 茶叶科学, 2026 , 46(2) : 318 -330 . DOI: 10.13305/j.cnki.jts.2026.02.007

Abstract

In June and July 2024, two Fusarium spp. strains were isolated from blighted leaves of tea plants (Camellia sinensis) in Motuo County, Linzhi City, Xizang, China. Species identification was conducted using morphological characteristics, multi-locus phylogenetic analysis, and Koch's postulates. The results identified the strains as Fusarium dracaenophilum and Fusarium asiaticum. Pathogenicity tests confirm that both species induce tea leaf blight. In varietal pathogenicity assays, ‘Zhonghuang 1’ exhibited significantly stronger resistance to both pathogens than ‘Fuding Dabaicha’. The results of the biological characterization reveal that F. asiaticum exhibits optimal growth conditions at temperatures ranging from 20 ℃ to 25 ℃ and at pH levels between 7 and 9. The presence of sucrose, maltose, lactose, and yeast extract has been demonstrated to stimulate F. asiaticum growth. F. dracaenophilum exhibits optimal growth at 25 ℃ and pH 7, with lactose and yeast extract being the most favorable nutrients. These findings enhanced understanding of Fusarium pathogen diversity and provided a theoretical basis for managing foliar diseases in tea plantations in Xizang.

Key words： Fusarium spp.; morphological identification; multilocus phylogenetic analysis; tea leaf blight

参考文献

[1] Mukhopadhyay M, Mondal T K, Chand P K.Biotechnological advances in tea (Camellia sinensis [L.] O. Kuntze): a review[J]. Plant Cell Reports, 2015, 35(2): 255-287.
[2] Jeyaraj A, Chandran V, Gajjeraman P.Differential expression of microRNAs in dormant bud of tea [Camellia sinensis (L.) O. Kuntze][J]. Plant Cell Reports, 2014, 33(7): 1053-1069.
[3] Tang G Y, Meng X, Gan R Y, et al.Health functions and related molecular mechanisms of tea components: an update review[J]. International Journal of Molecular Sciences, 2019, 20(24): 6196. doi: 10.3390/ijms20246196.
[4] 尤星宇, 冷杨, 霍春悦, 等. 2024年中国茶叶进出口贸易情况简析[J]. 中国茶叶, 2025, 47(3): 14-19.
You X Y, Leng Y, Huo C Y, et al.A brief analysis of tea import and export trade in China in 2024[J]. China Tea, 2025, 47(3): 14-19.
[5] Chen P F, Cai J R, Zheng P, et al.Quantitatively unravelling the impact of high altitude on oolong tea flavor from Camellia sinensis grown on the plateaus of Tibet[J]. Horticulturae, 2022, 8(6): 539. doi: 10.3390/horticulturae8060539.
[6] Hu G S, Wan M Z, Wei K, et al.Computer vision based method for severity estimation of tea leaf blight in natural scene images[J]. European Journal of Agronomy, 2023, 144: 126756. doi: 10.1016/j.eja.2023.126756.
[7] Shahriar S A, Nur-Shakirah A O, Mohd M H. Neopestalotiopsis clavispora and Pseudopestalotiopsis camelliae-sinensis causing grey blight disease of tea (Camellia sinensis) in Malaysia[J]. European Journal of Plant Pathology, 2021, 162(3): 709-724.
[8] Liu F, Ma Z Y, Hou L W, et al.Updating species diversity of Colletotrichum, with a phylogenomic overview[J]. Studies in Mycology, 2022, 101: 1. doi: 10.3114/sim.2022.101.01.
[9] Jeyaraj A, Elango T, Chen X, et al.Advances in understanding the mechanism of resistance to anthracnose and induced defence response in tea plants[J]. Molecular Plant Pathology, 2023, 24(10): 1330-1346.
[10] Guo M J, Zhao S Y, Gao Y, et al.A phylogenetic and taxonomic revision of Discula theae-sinensis, the causal agents of anthracnose on Camellia sinensis[J]. Journal of Fungi, 2024, 10(2): 141. doi: 10.3390/jof10020141.
[11] Crous P, Lombard L, Sandoval-Denis M, et al. Fusarium: more than a node or a foot-shaped basal cell[J]. Studies in Mycology, 2021, 98: 100106. doi: 10.1016/j.simyco.2021.100116.
[12] Sandoval-Denis M, Costa M M, Broders K, et al.An integrative re-evaluation of the Fusarium sambucinum species complex[J]. Studies in Mycology, 2025, 110: 1. doi: 10.3114/sim.2025.110.01.
[13] Xu W, Zhao F H, Deng X X, et al.First report of collar canker and dieback of Camellia sinensis caused by Fusarium solani species complex in Henan, China[J]. Plant Disease, 2022, 106(8): 2263. doi: 10.1094/pdis-07-21-1517-pdn.
[14] Zhang X Y, Yi J, Huo D, et al.First report of Fusarium proliferatum causing leaf spot on tea plants in Jiangxi Province, China[J]. Plant Disease, 2023, 107(7): 2220. doi: 10.1094/pdis-04-22-0896-pdn.
[15] 王文华. 墨脱县冬季气候与热带果树引种适应性[J]. 热带农业科学, 2020, 40(9): 39-44.
Wang W H.Adaptability of introduced tropical fruit trees to winter in Motuo County[J]. Chinese Journal of Tropical Agriculture, 2020, 40(9): 39-44.
[16] 朱罗娟, 江冰冰, 马玉青, 等. 西双版纳茶区茶轮斑病的致病病原分离及鉴定[J]. 茶叶科学, 2025, 45(6): 1047-1054.
Zhu L J, Jiang B B, Ma Y Q, et al.Isolation and identification of pathogens causing tea gray blight in Xishuangbanna tea area[J]. Journal of Tea Science, 2025, 45(6): 1047-1054.
[17] O'donnell K, Kistler H, Cigelnik E, et al. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies[J]. PNAS, 1998, 95(5): 2044-2049.
[18] Liu Y J, Whelen S, Hall B.Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse Ⅱ subunit[J]. Molecular Biology and Evolution, 1999, 16(12): 1799-1808.
[19] Reeb V, Lutzoni F, Roux C.Contribution of RPB2 to multilocus phylogenetic studies of the euascomycetes (Pezizomycotina, Fungi) with special emphasis on the lichen-forming Acarosporaceae and evolution of polyspory[J]. Molecular Phylogenetics and Evolution, 2004, 32(3): 1036-1060.
[20] O'donnell K, Sutton D A, Rinaldi M G, et al. Internet-accessible DNA sequence database for identifying Fusaria from human and animal infections[J]. Journal of Clinical Microbiology, 2010, 48(10): 3708-3718.
[21] O'donnell K, Nirenberg H, Aoki T, et al. A multigene phylogeny of the Gibberella fujikuroi species complex: detection of additional phylogenetically distinct species[J]. Mycoscience, 2000, 41: 61-78. doi: 10.1007/BF02464387.
[22] Han S L, Wang M M, Ma Z Y, et al. Fusarium diversity associated with diseased cereals in China, with an updated phylogenomic assessment of the genus[J]. Studies in Mycology, 2023, 104(1): 87-148.
[23] Zeng X Y, Tan T J, Tian F H, et al.OFPT: a one-stop software for fungal phylogeny[J]. Mycosphere, 2023, 14(1): 1730-1741.
[24] Katoh K, Standley D M.MAFFT multiple sequence alignment software version 7: improvements in performance and usability[J]. Molecular Biology and Evolution, 2013, 30(4): 772-780.
[25] Capella-Gutiérrez S, Silla-Martínez J M, Gabaldón T. TrimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses[J]. Bioinformatics, 2009, 25(15): 1972-1973.
[26] Kalyaanamoorthy S, Minh B Q, Wong T K F, et al. ModelFinder: fast model selection for accurate phylogenetic estimates[J]. Nature Methods, 2017, 14(6): 587-589.
[27] Nguyen L T, Schmidt H A, Von Haeseler A, et al.IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies[J]. Molecular Biology and Evolution, 2015, 32(1): 268-274.
[28] Bao Y M, Zhang Z, Zhao W M, et al.Database resources of the national genomics data center, China national center for bioinformation in 2025[J]. Nucleic Acids Research, 2024, 53(D1): D30-D44.
[29] Bu C F, Zheng X C, Zhao X T, et al. GenBase: a nucleotide sequence database [J]. Genomics, Proteomics & Bioinformatics, 2024, 22(3): qzae047. doi: 10.1093/gpbjnl/qzae047.
[30] 张婷, 王艳, 晋玲, 等. 当归根腐病主要病原菌生物学特性及室内毒力测定[J]. 微生物学杂志, 2024, 44(2): 71-78.
Zhang T, Wang Y, Jin L, et al.Biological characteristics and indoor toxicity test of main pathogens of Chinese Angelica (Angelica sinensis) root rot[J]. Journal of Microbiology, 2024, 44(2): 71-78.
[31] 靳启明, 闫成才, 王庆朋, 等. 6株核桃腐烂病菌的分子鉴定及生物学特性比较[J]. 山东农业科学, 2024, 56(12): 98-104.
Jin Q M, Yan C C, Wang Q P, et al.Molecular identification and comparison on biological characteristics of six walnut canker pathogens[J]. Shangdong Agricultural Science, 2024, 56(12): 98-104.
[32] 谢江涛, 覃再丽, 刘雪, 等. 毛木耳蛛网病致病菌生物学特性及杀菌剂敏感性分析[J]. 菌物学报, 2024, 43(8): 16-25.
Xie J T, Qin Z L, Liu X, et al.Biological characteristics and fungicide sensitivities of Hypomyces mycophilus causing cobweb disease of Auricularia cornea[J]. Mycosystema, 2024, 43(8): 16-25.
[33] Manawasinghe I S, Phillips A J L, Xu J P, et al. Defining a species in fungal plant pathology: beyond the species level[J]. Fungal Diversity, 2021, 109(1): 267-282.
[34] Manawasinghe I S, Jayawardena R S, Li H L, et al.Microfungi associated with Camellia sinensis: a case study of leaf and shoot necrosis on tea in Fujian, China[J]. Mycosphere, 2021, 12(1): 430-518.
[35] Fan Q, Su P P, Xiao J C, et al.Phylogenomic, morphological, and phylogenetic evidence reveals five new species and two new host records of Nectriaceae (Hypocreales) from China[J]. Biology, 2025, 14(7): 871. doi: 10.3390/biology14070871.
[36] 毛娟, 旺杰次仁, 罗英, 等. 林芝茶园土壤养分特征与肥力质量评价[J]. 江苏农业科学, 2023, 51(23): 211-218.
Mao J, Wangjie C R, Luo Y, et al.Soil nutritional status analysis and fertility assessment of tea plantations in Linzhi region[J]. Jiangsu Agricultural Sciences, 2023, 51(23): 211-218.
[37] 史田斌, 王多锋, 张露荷, 等. 陇南地区8个茶品种品质评价[J]. 甘肃农业大学学报, 2024, 59(6): 309-317.
Shi T B, Wang D F, Zhang L H, et al.Quality assessment of eight tea varieties in Longnan region[J]. Journal of Gansu Agricultural University, 2024, 59(6): 309-317.
[38] Sánchez-Hernández E, Andrés-Juan C, Buzón-Durán L, et al.Antifungal activity of methylxanthines against grapevine trunk diseases[J]. Agronomy, 2022, 12(4): 885. doi: 10.3390/agronomy12040885.
[39] Kurek J, Sierakowska A, Berdzik N, et al. Colchicine, caffeine, gramine, and their derivatives as potential herbicides, fungicides, and insecticides[J]. International Journal of Molecular Sciences, 2024, 25(18): 10081. doi: 10.3390/ijms251810081.
[40] Zhang Y Q, Wang F Y, Wang L J, et al.The response of growth and transcriptome profiles of tea grey blight disease pathogen Pestalotiopsis theae to the variation of exogenous L-theanine[J]. International Journal of Molecular Sciences, 2024, 25(6): 3493. doi: 10.3390/ijms25063493.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献