使用基于EthoVision XT11.5软件的视频轨迹分析仪检测了交尾和未交尾的雌、雄小贯小绿叶蝉成虫对茶鲜叶和黄、白、绿色卡的搜寻行为,结果发现：（1）未交尾雌成虫从释放点缓慢地搜寻到叶片,大多时间在叶片之外区域活动;交尾雌成虫从释放点较快地搜寻到叶片,较多时间在叶片上活动;（2）未交尾雄成虫从释放点较快地搜寻到叶片,大多时间在叶片上搜寻,轨迹长而复杂且速度快;交尾雄成虫较多时间在叶片之外区域搜寻,速度稍慢;（3）受到刺激时试虫就会展翅,致体翅面积增大,大于正常体翅面积60%、20%~60%、20%以下的运动状态分别称为“狂躁”、“活跃”和“静止”行为,交尾后雌、雄成虫“狂躁”历时均显著长于交尾前;（4）交尾前或交尾后,在黄色卡上雌成虫搜寻历时长于雄成虫,而在绿色卡上雄成虫搜寻历时长于雌成虫;（5）交尾之后,雌虫在黄卡区域出现频率和搜寻时间皆略有增加,在绿卡区域出现频率和搜寻时间皆明显减少;相应地,雄虫在黄卡、绿卡区域出现频率和停留时间皆略有减少。分析认为：交尾前,雄虫对茶叶的趋性强于雌虫;交尾后,雌虫对于叶片气味定向性增加,而雄虫对叶片气味的偏好性稍减弱;雄虫稍嗜好绿色,雌虫稍嗜好黄色;交尾稍增强了雌虫对黄色的嗜好,略降低了雄虫对于绿色的嗜好。

The searching behavior of Empoasca onukii for fresh tea leaves and color cards in yellow, white and green was investigated by the video trajectory analysis instrument, which was based on EthoVision XT 11.5 software. The results showed that: (1) it took more time for virgin females to move from the releasing spot to the tested leaf edge. They mostly moved out the experimental leaf, while the copulated females moved from the releasing spot to the tested leaf edge more quickly and spent the most of time on the experimental leaf. (2) The virgin males detected the leaf from the releasing spot quickly, whose trajectory was very long, complicated and mostly on the leaf, and the copulated males slightly slowed down their speed, mostly searched out the leaf. (3) If stimulated, the tested leafhopper would open out the wings and result in the increase of its body area. When surpassed the normal area by 60%, 20% to 60 % and lower than 20%, the leafhopper′s status was called ′mania′, ′activity′ and ′still′ behavior respectively. After copulating, the ′mania′ durations of both female and male significantly lengthened, especially male. (4) Both before and after copulating, the female spent more time on the yellow card than the male; however, on the green card, the male searched for a longer time than the female. (5) After copulating, both emerging frequency and searching time of females slightly rose within yellow card area, but obviously dropped within green card area. Correspondingly, both emerging frequency and searching time of males a little reduced within both yellow and green cards. It was considered that before copulating, males were more intensively taxised to tea leaf than females, but after copulating, the directionality of females to the odor from tea leaf increased but the preference of males for the odor from tea leaf weakened. Moreover, males preferred green slightly and females preferred yellow, and copulation enhanced the preference of females to yellow but reduced that of males to green.