Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography-Evaporative Light-Scattering Detector

LI Yin-hua; LIU Zhong-hua&#x0002A;; HUANG Jian-an; GONG Yu-shun

doi:10.13305/j.cnki.jts.2005.03.012

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Journal of Tea Science ›› 2005, Vol. 25 ›› Issue (3) : 225-228. DOI: 10.13305/j.cnki.jts.2005.03.012

Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography-Evaporative Light-Scattering Detector

LI Yin-hua, LIU Zhong-hua*, HUANG Jian-an, GONG Yu-shun

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Abstract

High performance liquid chromatography (HPLC) coupled with evaporative light scattering detector（ELSD-2000）was used for the determination of theanine in tea without derivatization. A Polaris-C₁₈ column (250βmm×4.6βmm) was used with the mobile phase of 0.1%trifluoroacetic acid water solution and 0.1% trifluoroacetic acid acetonitrile solution .The flow rate was at 0.5βml/min. The linear response ranged from 0.062~0.450βmg/mL for theanine. The recovery was 97.5%. The method was evaluated as accurate and simple.

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Evaporative light-scattering detector / HPLC / Tea / Theanine

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LI Yin-hua, LIU Zhong-hua*, HUANG Jian-an, GONG Yu-shun. Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography-Evaporative Light-Scattering Detector[J]. Journal of Tea Science. 2005, 25(3): 225-228 https://doi.org/10.13305/j.cnki.jts.2005.03.012

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