HPLC Analysis, Preparation and Mass Identification of Flavonol Glycosides in Tea Seed Flakes

JIANG He-yuan; KE Chang-qiang; WANG Chuan-pi; CHEN Xiao-qiang; YUAN Xin-yue

doi:10.13305/j.cnki.jts.2005.04.009

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Journal of Tea Science ›› 2005, Vol. 25 ›› Issue (4) : 289-294. DOI: 10.13305/j.cnki.jts.2005.04.009

HPLC Analysis, Preparation and Mass Identification of Flavonol Glycosides in Tea Seed Flakes

JIANG He-yuan¹, KE Chang-qiang², WANG Chuan-pi¹, CHEN Xiao-qiang¹, YUAN Xin-yue¹

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Abstract

Through HPLC and preparing HPLC and the analysis of molecular weight and chemical group by HPLC/MS, Two flavonol glycosides from tea seed flakes were separated and identied. The systematical method combining analytical and preparation scale HPLC with HPLC/MS, could also be used in the study of other natural products in tea.

Key words

Flavonol glycosides / HPLC / LC/MS / Tea seed flakes

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JIANG He-yuan, KE Chang-qiang, WANG Chuan-pi, CHEN Xiao-qiang, YUAN Xin-yue. HPLC Analysis, Preparation and Mass Identification of Flavonol Glycosides in Tea Seed Flakes[J]. Journal of Tea Science. 2005, 25(4): 289-294 https://doi.org/10.13305/j.cnki.jts.2005.04.009

References

[1] 柳荣祥, 朱全芬, 夏春华. 茶皂素表面活性剂及其应用研究进展[J]. 日用化学工业, 1996(5): 32~36.
[2] M Amoros, L Girre.Mechanism of antiviral activity of triterpenoid saponins[J]. Phytotherapy Research, 1999, 13(4): 323~328.
[3] M GUO, F SONG.Rapid Analysis of a Triterpenoid Saponin Mixture from Plant Extracts by Electrospray Ionization Multi-stage Tandem Mass Spectrometry (ESI-MSn)[J]. Analytical Sciences. 2002, 18(4): 481~484.
[4] Ferran S R, Olga J, Isidre C, et al. Liquid chromatographic/ electrospray ionization tandem mass spectrometric study of the phenolic composition of cocoa (Theobroma cacao)[J]. Mass Spectrom., 2003, 38: 35~42.
[5] Hajime S, Masazum M, Susumu I, et al.1993, Saponins from leaves of Acanthopanax Sieboldianus[J]. Phytochemistry, 34(4): 1117~1121.
[6] 贾世山, 马超美, 李英和, 等. 甘草叶中酚酸和黄酮甙类成分的分离鉴定[J]. 药学学报, 1992, 27(6): 441~444.
[7] Masao Y, Hiroharu F, Mihoko M, et al. A chemotaxonomic study of flavonoids in the leaves of six Trichosanthes[J] species. Phytochemistry, 1987, 26(9): 2557~2558.