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Journal of Tea Science ›› 2007, Vol. 27 ›› Issue (1): 33-38.doi: 10.13305/j.cnki.jts.2007.01.005

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Anticancer Activity of Theaflavin Diagallate and its Mechanism

JIANG He-yuan1, 2, Hang Xiao2, YUAN Xin-yue1, WANG Chuan-pi1, Chung S Yang2   

  1. 1. Tea Research Institute, Chinese Academy of Agriculture Sciences, Zhejiang, Hangzhou 310008, China;
    2. Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, NJ08854, USA
  • Received:2006-07-27 Revised:2006-09-27 Online:2007-03-25 Published:2019-09-11

Abstract: Theaflavin digallate was purified by Sephadex LH-20 column chromatography. Its anticancer activity and mechanism were analyzed on H1299 and HCT-116 cancer cell lines. Results from MTT assay showed that Theaflavin digallate could inhibit the growth of H1299, and its IC50 value is 25μmol/L. Theaflavin digallate could slightly adjust the cell cycle of HCT-116 cancer cell, and significantly promote the apoptosis of HCT-116 cancer cell at 50μmol/L. Theaflavin digallagte could decrease the expression of cancer-promoting biomarker, Bcl-xL, and increase the expression of cancer-inhibiting biomarker, Bax, on HCT-116 cancer cell line.

Key words: theaflavin, anticancer, cell cycle, apoptosis, western blotting

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