Effect of SOD on Anti-cancer Activity and Stability of Epigallocatechin-3-gallate

ZHANG Bin; XIA Yan-qiu; JIN Li-ji; XU Yong-ping

doi:10.13305/j.cnki.jts.2007.04.006

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Journal of Tea Science ›› 2007, Vol. 27 ›› Issue (4) : 293-298. DOI: 10.13305/j.cnki.jts.2007.04.006

Effect of SOD on Anti-cancer Activity and Stability of Epigallocatechin-3-gallate

ZHANG Bin, XIA Yan-qiu, JIN Li-ji, XU Yong-ping

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Abstract

Effects of epigallocatechin-3-gallate (EGCG) on the growth of human colonic cancer cells HT-29, human breast cancer MCF-7 and human cervical cancer Hela were investigated by MTT assay. Cytotoxicity of EGCG on these cells was detected by determining the activity of LDH in culture medium. Induction of apoptosis in HT-29 cell after the EGCG treatment was analyzed by flow cytometry. Stability of EGCG in DMEM/F-12 was determined by HPLC and all studies above were compared in the presence or absence of SOD. Investigation showed that cells survival percentage deceased and activity of LDH in the culture medium increased after adding SOD. The apoptotic rate of HT-29 and the stability of EGCG in culture increased in the presence of SOD comparing to absence of SOD.

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EGCG / HT-29 cells / SOD

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ZHANG Bin, XIA Yan-qiu, JIN Li-ji, XU Yong-ping. Effect of SOD on Anti-cancer Activity and Stability of Epigallocatechin-3-gallate[J]. Journal of Tea Science. 2007, 27(4): 293-298 https://doi.org/10.13305/j.cnki.jts.2007.04.006

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