Separation, Purification and Structure Identification of Flavonol Glycosides in Black Tea

GAO Qing-qing; JIANG He-yuan; ZHANG Jian-yong; YUAN Xin-yue; KOU Xiao-hong; GAO Qi; LIU Xiao-hui; KE Chang-qiang

doi:10.13305/j.cnki.jts.2008.04.003

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Journal of Tea Science ›› 2008, Vol. 28 ›› Issue (4) : 277-281. DOI: 10.13305/j.cnki.jts.2008.04.003

Separation, Purification and Structure Identification of Flavonol Glycosides in Black Tea

GAO Qing-qing^1,2, JIANG He-yuan^1,*, ZHANG Jian-yong¹, YUAN Xin-yue^1,2, KOU Xiao-hong¹, GAO Qi¹, LIU Xiao-hui^1,2, KE Chang-qiang³

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Abstract

Column chromatography, HPLC and prepared HPLC were used together to separate and purify the ethyl acetate extract of Dajielee black tea, and the mixture of two flavonol glycosides was obtained. The molecule NMR methods weights and structure of these two flavonol glycosides were identified by using the HPLC/MS and NMR methods.

Key words

black tea / Flavonol glycosides / HPLC / LC/MS / NMR

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GAO Qing-qing, JIANG He-yuan, ZHANG Jian-yong, YUAN Xin-yue, KOU Xiao-hong, GAO Qi, LIU Xiao-hui, KE Chang-qiang. Separation, Purification and Structure Identification of Flavonol Glycosides in Black Tea[J]. Journal of Tea Science. 2008, 28(4): 277-281 https://doi.org/10.13305/j.cnki.jts.2008.04.003

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