Determination of Theanine in Tea by High Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry

Frank Sen-Chun Lee; WANG Hong; KANG Hai-ning; CHEN Jun-hui; ZHAO Heng-qiang; WANG Xiao-ru

doi:10.13305/j.cnki.jts.2009.1.004

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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (1) : 20-26. DOI: 10.13305/j.cnki.jts.2009.1.004

Determination of Theanine in Tea by High Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry

WANG Hong^1,3, KANG Hai-ning^1,2, CHEN Jun-hui^1,*, ZHAO Heng-qiang¹, WANG Xiao-ru^1,2, Frank Sen-Chun Lee¹

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Abstract

HPLC coupled online with atmospheric pressure chemical ionization MS technique was developed for the qualitative and quantitative determination of theanine in tea. The theanine and other compounds in the tea extract were separated on an Alltima C₈ column with methanol and water (10:90 v/v) as mobile phase, with flow rate of 0.6 ml/min and APCI-MS SIM m/z 175.1 detection. In the positive APCI mode, sensitive and stable MS signal could be obtained by APCI-MS (SIM m/z 175.1) detection, and the APCI-MS conditions were also optimized by orthogonal design. The results indicated that HPLC-APCI-MS method was simple, sensitive and reliable for the determination of theanine in tea. It was important that the detection sensitivity was improved greatly by APCI-MS than UV. Eight tea samples from Fujian province were analysed using the established methods.

Key words

HPLC-APCI-MS / orthogonal experiment / SIM mode / tea / theanine

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WANG Hong, KANG Hai-ning, CHEN Jun-hui, ZHAO Heng-qiang, WANG Xiao-ru, Frank Sen-Chun Lee. Determination of Theanine in Tea by High Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry[J]. Journal of Tea Science. 2009, 29(1): 20-26 https://doi.org/10.13305/j.cnki.jts.2009.1.004

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