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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (1): 67-71.doi: 10.13305/j.cnki.jts.2009.1.011

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A Method for Determination of Imidacloprid Residue in Tea with HPLC-UV

HOU Ru-yan, ZHU Xu-jun, ZHANG Zheng-zhu, CAI Hui-mei, WAN Xiao-chun*   

  1. Key Laboratory of Tea biochemistry & biotechnology, Ministry of Agriculture, PRC, Anhui Agricultural University, Hefei 230036, China
  • Received:2008-03-31 Revised:2008-11-05 Online:2009-02-15 Published:2019-09-06

Abstract: A method was developed for determination of imidacloprid residue in tea by high performance liquid chromatography (HPLC) with UV detector. Imidacloprid in tea samples was extracted with methyl alcohol and re-extracted by dichloromethane then cleaned up with the ENVI-18 cartridge. Imdacloprid was eluted with acetonitrile:water(v/v, 30:70) solution from the cartridge and detected with HPLC. The recovery of imidacolprid ranged from 81.5%~87.9% with the RSD less than 10.2%. Practical determination limit was 0.05 mg/kg.

Key words: tea, imidacloprid, pesticide residues, HPLC

CLC Number: