Cloning and Expression Analysis on Citrate Synthase Gene of Tea Root under Low Phosphorous Stress

LIN Zheng-he; CHEN Chang-song; WU Ling-sheng

doi:10.13305/j.cnki.jts.2010.05.006

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Journal of Tea Science ›› 2010, Vol. 30 ›› Issue (5) : 362-366. DOI: 10.13305/j.cnki.jts.2010.05.006

Cloning and Expression Analysis on Citrate Synthase Gene of Tea Root under Low Phosphorous Stress

LIN Zheng-he, CHEN Chang-song, WU Ling-sheng

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Abstract

Citrate synthase(CS) is the key enzyme of producing citrate acid. CS gene fragments were cloned from tea roots. The GenBank accession is FJ814766 in the NCBI. Then the changes in gene expression in response to P supply were investigated by using semi-quantitative RT-PCR. The result showed that P deficiency increased the expressions of CS gene. This may be a tolerant mechanism of tea seedlings to P deficiency.

Key words

cloning / gene expression / low P stress / semi-quantitative RT-PCR / tea plant

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LIN Zheng-he, CHEN Chang-song, WU Ling-sheng. Cloning and Expression Analysis on Citrate Synthase Gene of Tea Root under Low Phosphorous Stress[J]. Journal of Tea Science. 2010, 30(5): 362-366 https://doi.org/10.13305/j.cnki.jts.2010.05.006

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