A Method for Determination of Imidacloprid Residue in Tea with HPLC-UV

WANG Xiao-hui; WAN Xiao-chun; HOU Ru-yan

doi:10.13305/j.cnki.jts.2012.03.008

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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (3) : 203-209. DOI: 10.13305/j.cnki.jts.2012.03.008

A Method for Determination of Imidacloprid Residue in Tea with HPLC-UV

WANG Xiao-hui, WAN Xiao-chun, HOU Ru-yan^*

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Abstract

A method for the analysis of imidacloprid residue in tea by high performance liquid chromatography (HPLC) with UV detector was established. Through the comparation of extract efficiencies of several solvent and the clean-up capacities of different SPE cartridges, the results show that after soaked with water, extracted by acetonitrile, cleaned up with the Envi-Carb/PSA cartridge, imidacloprid was eluted with 6mL acetonitrile solution from the cartridge and detected with HPLC, the samples was spiked at the level of 0.025~0.250mg/kg, and the recovery of Imidacloprid range from 92.0%~97.6% with RSD less than 3.8%. Practical determination limit was 0.025mg/kg. It meet with the analytical requirements to imidacloprid residue in exported tea. The pretreatment method was simple, sensitive and less organic solvent consumption.

Key words

HPLC / midacloprid / pesticide residues / tea

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WANG Xiao-hui, WAN Xiao-chun, HOU Ru-yan. A Method for Determination of Imidacloprid Residue in Tea with HPLC-UV[J]. Journal of Tea Science. 2012, 32(3): 203-209 https://doi.org/10.13305/j.cnki.jts.2012.03.008

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