Isolation and Identification of EGCG3″Me and EGCG4″Me from Tea

LI Yin-hua; ZHANG Sheng; HUANG Jian-an; LIU Zhong-hua

doi:10.13305/j.cnki.jts.2012.04.005

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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (4) : 313-318. DOI: 10.13305/j.cnki.jts.2012.04.005

Isolation and Identification of EGCG3″Me and EGCG4″Me from Tea

LI Yin-hua, ZHANG Sheng, HUANG Jian-an, LIU Zhong-hua^*

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Abstract

：A method was established to isolate EGCG3"Me and EGCG 4"Me by using column chromatography and preparative high performance liquid chromatography. Crude extracts was obtained from tea leaves by using ethyl acetate and water as solvent .Then the concentracted crude extract was separated on a HP-20 column. The Fr-1 fraction was obtained from the eluate of 80% ethanol and subjected to a preparative high performance liquid chromatography for isolation of target components by using water-acetonitrile as mobile phase. Two compounds were identified with ultraviolet absorbance(UV), nuclear magnetic resonance(NMR), mass spectrometry(MS). They were EGCG3"Me and EGCG4"Me.The purity of them was more than 98%.

Key words

EGCG3"Me / EGCG4"Me / isolation and preparation / tea

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LI Yin-hua, ZHANG Sheng, HUANG Jian-an, LIU Zhong-hua. Isolation and Identification of EGCG3″Me and EGCG4″Me from Tea[J]. Journal of Tea Science. 2012, 32(4): 313-318 https://doi.org/10.13305/j.cnki.jts.2012.04.005

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