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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (5): 441-447.doi: 10.13305/j.cnki.jts.2012.05.001

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Study on the Reaction Condition of EGCG Methylated Derivatives Produced from EGCG Catalyzed by EGCG-O-Methyltransferase

LV Hai-peng, FEI Dong-mei, ZHANG Yue, LIN Zhi*, PENG Qun-hua, GUO Li, TAN Jun-feng   

  1. National Engineering Research Center for Tea Industry, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2012-03-07 Revised:2012-03-31 Online:2012-10-15 Published:2019-09-05

Abstract: Taken the yields of EGCG3"Me, EGCG4′′Me and EGCG3′Me as the main index, the investigation on the reaction condition of EGCG methylated derivatives produced from EGCG catalyzed by EGCG-O-Methyltransferase were conducted. Results showed that the optimum reaction condition was as follows: the concentration of DTT and Mg2+ was 2mmol/L, the initial pH was 7.5 and the reaction temperature was 35℃. Under the above conditions, the yields of EGCG3"Me, EGCG4′′Me and EGCG3′Me could reach 386.39μg/mL, 23.40μg/mL and 107.01μg/mL, respectively.

Key words: EGCG methylated derivatives, enzymatic synthesis, EGCG-O-Methyltransferase

CLC Number: