Study on the Reaction Condition of EGCG Methylated Derivatives Produced from EGCG Catalyzed by EGCG-O-Methyltransferase

LV Hai-peng; FEI Dong-mei; ZHANG Yue; LIN Zhi; PENG Qun-hua; GUO Li; TAN Jun-feng

doi:10.13305/j.cnki.jts.2012.05.001

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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (5) : 441-447. DOI: 10.13305/j.cnki.jts.2012.05.001

Study on the Reaction Condition of EGCG Methylated Derivatives Produced from EGCG Catalyzed by EGCG-O-Methyltransferase

LV Hai-peng, FEI Dong-mei, ZHANG Yue, LIN Zhi^*, PENG Qun-hua, GUO Li, TAN Jun-feng

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Abstract

Taken the yields of EGCG3"Me, EGCG4′′Me and EGCG3′Me as the main index, the investigation on the reaction condition of EGCG methylated derivatives produced from EGCG catalyzed by EGCG-O-Methyltransferase were conducted. Results showed that the optimum reaction condition was as follows: the concentration of DTT and Mg²⁺ was 2mmol/L, the initial pH was 7.5 and the reaction temperature was 35℃. Under the above conditions, the yields of EGCG3"Me, EGCG4′′Me and EGCG3′Me could reach 386.39μg/mL, 23.40μg/mL and 107.01μg/mL, respectively.

Key words

EGCG methylated derivatives / EGCG-O-Methyltransferase / enzymatic synthesis

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LV Hai-peng, FEI Dong-mei, ZHANG Yue, LIN Zhi, PENG Qun-hua, GUO Li, TAN Jun-feng. Study on the Reaction Condition of EGCG Methylated Derivatives Produced from EGCG Catalyzed by EGCG-O-Methyltransferase[J]. Journal of Tea Science. 2012, 32(5): 441-447 https://doi.org/10.13305/j.cnki.jts.2012.05.001

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