The Gene Cloning and Expression Analysis of UFGT in Tea Plant [Camellia sinensis (L.) O. Kuntze]

WANG Xiao-fan; TIAN Yan-wei; WANG Yun-sheng; GAO Li-ping; XIA Tao

doi:10.13305/j.cnki.jts.2012.05.010

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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (5) : 411-418. DOI: 10.13305/j.cnki.jts.2012.05.010

The Gene Cloning and Expression Analysis of UFGT in Tea Plant [Camellia sinensis (L.) O. Kuntze]

WANG Xiao-fan¹, TIAN Yan-wei², WANG Yun-sheng², GAO Li-ping^2*, XIA Tao^1*

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Abstract

A glucosyltransferase gene UDP-flavonoid 3-O-glucosyl transferase was isolated from tea plant [Camellia sinensis (L.) O. Kuntze] and named CsUFGT. CsUFGT has 1526bp full length with open reading frame of 1380bp which encodes 459 amino acids. The corresponding protein CsUFGT, with predicted molecular mass 49.486kDa and predicted isoelectric point 5.96, shares 59% identity and 75% similarity with UFGT(P51094.2) in Vitis vinifer. CsUFGT includes a PSPG signal motif of typical plant glucosyltransferase. qRT-PCR analysis showed that the gene expressed in all tissues of tea plant [Camellia sinensis (L.) O. Kuntze], and had high expression in the fourth leaf and low expression level in root and stem.

Key words

Camellia sinensis / expression analysis / flavonoid 3-O-glucosyl transferase / gene cloning

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WANG Xiao-fan, TIAN Yan-wei, WANG Yun-sheng, GAO Li-ping, XIA Tao. The Gene Cloning and Expression Analysis of UFGT in Tea Plant [Camellia sinensis (L.) O. Kuntze][J]. Journal of Tea Science. 2012, 32(5): 411-418 https://doi.org/10.13305/j.cnki.jts.2012.05.010

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