Identification and Phylogenetic Analysis of Anthracnose Pathogen Colletotrichum fructicola Isolated from Camellia sinensis

LIU Wei; YE Naixing; CHEN Yusen; LIAN Lingli; LIU Wei; JIN Shan; LAI Jiandong; XIE Yunhai

doi:10.13305/j.cnki.jts.2014.01.012

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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (1) : 95-104. DOI: 10.13305/j.cnki.jts.2014.01.012

Identification and Phylogenetic Analysis of Anthracnose Pathogen Colletotrichum fructicola Isolated from Camellia sinensis

LIU Wei^1,2, YE Naixing^1,3,*, CHEN Yusen⁴, LIAN Lingli⁵, LIU Wei^3,6, JIN Shan^1,3, LAI Jiandong¹, XIE Yunhai¹

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Abstract

Eight strains of anthracnose pathogen, ZHG, WSX, ZTG, WHG, FTG, AHD, WRG and SLH were isolated from different tea cultivars in Fujian Province. These strains were identified by using morphological method and rDNA-ITS sequence analysis. The results showed that SLH strain was identified as Colletotrichum gloeosporioides, while the other seven strains were Colletotrichum fructicola, which has never been discovered on tea plant. The ITS sequences of C. fructicola and those of nine strains of anthracnose pathogen from tea-oil tree, jasmine plant and papaya (psidium guajave), were got for a clustering analysis. The results showed that base mutations or deletions were existed in the seven strains from tea plant, and the seven strains of C. fructicola were geneticly close to the pathogen C. gloeosporioides, isolated from tea plant, oil-tea plant and jasmine plant.

Key words

Colletotrichum fructicola / pathogen identification / phylogenetic analysis / rDNA-ITS / sequence analysis / tea plant(Camellia sinensis)

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LIU Wei, YE Naixing, CHEN Yusen, LIAN Lingli, LIU Wei, JIN Shan, LAI Jiandong, XIE Yunhai. Identification and Phylogenetic Analysis of Anthracnose Pathogen Colletotrichum fructicola Isolated from Camellia sinensis[J]. Journal of Tea Science. 2014, 34(1): 95-104 https://doi.org/10.13305/j.cnki.jts.2014.01.012

References

[1] 喻璋, 任国兰, 田尧甫, 等. 几个炭疽菌株的分类鉴定[J]. 河南农业大学报, 1996, 30(2): 149-153.
[2] 卢东升, 吴小芹. 豫南茶园树栖真菌分类研究[J]. 茶叶科学, 2004, 24(4): 243-248.
[3] 陈勇. 茶叶常见病害的发病症状及防治技术[J]. 现代农业科学, 2007, 21: 79-80.
[4] Hyde K D, Cai L, Cannon P F, et al. Colletotrichum names in current use[J]. Fungal Diversity, 2009, 39: 147-182.
[5] Hyde K D, Cai L, McKenzie E H C, et a1. Colletotrichum: a catalogue of confusion[J]. Fungal Diversity, 2009, 39: l-17.
[6] 曾大兴, 戚佩坤, 姜子德. 香蕉炭疽菌菌株亲缘关系的RAPD分析[J]. 菌物系统, 2001, 20(3): 324-329.
[7] 刘诗胤, 邱海萍, 姜华, 等. 浙江地区不同寄主来源的炭疽菌ITS序列PCR-RFLP多态性分析[J]. 浙江农业学报, 2012, 24(1): 6l-65.
[8] 曾大兴, 戚佩坤, 姜子德. 弯孢类炭疽菌rDNA ITS区的RFLP分析及分类研究[J]. 植物病理学报, 2004, 34(5): 431-436.
[9] Lu G Z, Paul F C, Alex R D, et al. Diversity and molecular relationships of endophytic Colletotrichum isolates from the Iwokrama Forest Reserve, Guyana[J]. MycolRes, 2004, 108(1): 53-63.
[10] 方中达. 植病研究方法[M]. 北京: 农业出版社, 1998: 179-210.
[11] 陈玉森, 叶乃兴, 许文耀, 等. 炭疽菌附着胞形成的影响因素及形态特征[J]. 福建农林大学学报: 自然科学版, 2013, 42(2): 137-142.
[12] 易克贤, 黄俊生, 刘国道. 中国柱花草炭疽病原菌遗传多态性的RAPD分析[J]. 微生物学报, 2003, 43(3): 379-387.
[13] White T J, Bruns T, Lee S, et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[C]. //Innis M A, Gelfand D H, Sninsky J J, et al. PCR Protocols: a guide to methods and applications. San Diego: Academic Press, 1990: 315-322.
[14] Arx J A V. Die Arten der Guttung Colletotrichum Corda[J]. Phytopathol Zeitschrift, 1957, 29: 413-468.
[15] Arx J A V. A revision of the fungi classified as Gloeosporium[J]. Bibliotheca Mycologica, 1970, 24: 170-180.
[16] Sutton B C.The Coelomycetes-fungi imperfecti with pycnidia, acervuli, and stromata[M]. London: Commonwealth Mycological Institute, 1980: 523-537.
[17] 陆家云. 植物病原真菌学[M]. 北京: 中国农业出版社, 2004: 455-458.
[18] 陆家云. 植物病害诊断[M]. 2版. 北京: 中国农业出版社, 1997: 181-186.
[19] 魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979: 495-476.
[20] Weir B S, Johnston P R, Damm U.The Colletotrichum gloeosporioides species complex[J]. Studies in mycology, 2012, 73: 115-180.
[21] Peng L J, Sun T, Yang Y L, et al. Colletotrichum species on grape in Guizhou and Yunnan provinces, China[J]. Mycoscience, 2013, 54: 29-41.
[22] Prihastuti H, Cai L, Chen H, et al. Characterisation of Colletotrichum species associated with coffee berries in northern Thailand[J]. Fungal Diversity, 2009, 39: 89-109.
[23] 陈宗懋, 陈雪芬. 茶树病害的诊断和防治[M]. 上海: 上海科学技术出版社, 1990: 32-41.
[24] 王晓鸣. 炭疽菌属(Colletotrichum Cda)的现代分类学和陕西省炭疽菌属的种[D]. 咸阳: 西北农业大学, 1985: 23-38.
[25] 刘守安, 韩宝瑜, 付建玉, 等. 茶炭疽病菌毒素的致病活性及理化性质初探[J]. 茶叶科学, 2007, 27(2): 153-158.
[26] 陆家云. 植物病害诊断[M]. 北京: 中国农业出版社, 1997: 186-187.
[27] 张中义, 刘云龙, 刘媛, 等. 中国炭疽菌属(Colletotrichum)的分类研究Ⅰ[J]. 石河子大学学报: 自然科学版, 2004, 22(7): 178-181.
[28] 谭济才. 茶树病害防治[M]. 北京: 中国农业出版社, 2002(6): 212-255.
[29] 杨友联. 中国贵州、云南、广西炭疽菌属真菌多基因分子系统学研究[D]. 武汉: 华中农业大学, 2010: 57-60.