Effect of Liupao Tea on Glucose and Lipid Metabolism in Palmitate-induced Insulin Resistance 3T3-L1 Adipocytes

TENG Cuiqin; LIU Zhonghua; GONG Shouji; PENG Yuxuan; Ma Rui

doi:10.13305/j.cnki.jts.2014.03.004

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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (3) : 230-238. DOI: 10.13305/j.cnki.jts.2014.03.004

Effect of Liupao Tea on Glucose and Lipid Metabolism in Palmitate-induced Insulin Resistance 3T3-L1 Adipocytes

TENG Cuiqin^1,2, LIU Zhonghua^1,2,*, GONG Shouji³, PENG Yuxuan⁴, Ma Rui⁵

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Abstract

To investigate the effect of Liupao tea on glucose and lipid metabolism in palmitate-induced insulin resistance 3T3-L1 adipocytes. Glucose intake decreased and insulin resistance was induced in 3T3-L1 adipocytes after incubation in 1mmol/L palmitate for 24 hours. Compared with the model group, the concentrations of glucose and NEFA decreased along with the increase of Liupao tea concentrations in medium. The mRNA expression of key enzyme for glycolysis of glucose (Hexokinase, 6-phosphofructokinase-1, and pyruvatekinase) and the Lipid-Metabolism-Related Enzymes (Sn-glycerol-3-phosphate acyltransferase) significantly increased in the Liupao tea groups comparing with model sample. Meanwhile the glucose transporter 4 mRNA expression was also increased and the acetyl-CoA carboxylase, protein tyrosine phosphatase 1B mRNA expression was decreased. Low doses of Liupao tea reduced carnitine palmitoyl transferase I mRNA expression while high doses significantly raised it. Research proved that Liupao tea were able to increase glucose uptake and activate the glucolipid metabolic pathways. Liupao tea showed the effect of improving insulin resistance induced by palmitate in 3T3 -L1 adipocytes.

Key words

3T3-L1 adipocytes / glucolipid metabolism / insulin resistance / Liupao tea

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TENG Cuiqin, LIU Zhonghua, GONG Shouji, PENG Yuxuan, Ma Rui. Effect of Liupao Tea on Glucose and Lipid Metabolism in Palmitate-induced Insulin Resistance 3T3-L1 Adipocytes[J]. Journal of Tea Science. 2014, 34(3): 230-238 https://doi.org/10.13305/j.cnki.jts.2014.03.004

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