Analysis and Structural Identification of Anthocyanin Components in Purple Buds and Leaves of Tea Plant

LI Zhi; WANG Riwei; ZHANG Lixia; HAN Xiaoyang

doi:10.13305/j.cnki.jts.2014.03.010

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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (3) : 279-287. DOI: 10.13305/j.cnki.jts.2014.03.010

Analysis and Structural Identification of Anthocyanin Components in Purple Buds and Leaves of Tea Plant

LI Zhi¹, WANG Riwei², ZHANG Lixia^1,*, HAN Xiaoyang²

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Abstract

A rapid method for analysis of anthocyanins in purple buds and leaves of tea plant was established by high-performance liquid chromatography (HPLC) based on the screening of mobile phase and the optimization of elution gradient. Using the established HPLC method, nine major chromatographic peaks were separated from crude extracts of anthocyanins from purple buds and leaves of tea plant. Further, the ultraviolet-visible spectrum and mass spectrum data of seven out the nine components were obtained by HPLC-DAD-MS/MS analysis. Combined with the literature data, five major components of anthocyanins were identified as delphindin-3-galactoside, cyanidin-3-gal-actoside, delphindin-3-rutinoside, cyanidin-3-rutinoside, and pelargonium-3-rutinoside. Additionally, each component was quantitatively analyzed using an external standard method, and crude extracts of anthocyanins were obtained at the purity of 32%. The proposed method is convenient and rapid with high accuracy and reproducibility, which can be applied for determining anthocyanins in purple buds and leaves of tea plant.

Key words

anthocyanins / component / HPLC / purple leaves of tea plant

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LI Zhi, WANG Riwei, ZHANG Lixia, HAN Xiaoyang. Analysis and Structural Identification of Anthocyanin Components in Purple Buds and Leaves of Tea Plant[J]. Journal of Tea Science. 2014, 34(3): 279-287 https://doi.org/10.13305/j.cnki.jts.2014.03.010

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