Cloning and Expression Analysis of CsbHLH2 in Tea Plant (Camellia sinensis)

HAN Yongtao; XIAO Bin; QIAN Wenjun; LIANG Shaoru

doi:10.13305/j.cnki.jts.2015.05.012

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Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (5) : 481-490. DOI: 10.13305/j.cnki.jts.2015.05.012

Cloning and Expression Analysis of CsbHLH2 in Tea Plant (Camellia sinensis)

HAN Yongtao, XIAO Bin^*, QIAN Wenjun, LIANG Shaoru

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Abstract

The bHLH (basic Helix-Loop-Helix) transcription factor is one of the most important transcription factor in plants, which play important roles in plant growth and stress regulation. The bHLH transcription factor CsbHLH2 was cloned from tea plant (Camellia sinensis) cultivars ‘Shanchayihao’ by homologous cloning technology using cDNA template. Bioinformatics analysis showed that the CsbHLH2 contains 714 bp ORF and was predicted encoding 297 amino acid, the deduced protein molecular weight was 58.4 kD and its theoretical isoelectric point was 5.14. Amino acid sequence alignment revealed that CsbHLH2 was highly homologous to other higher plant bHLH proteins. Transient expression of recombinant plasmid CsbHLH2/PBI221-GFP in Arabidopsis protoplasts showed that CsbHLH2 was located in cell nuclei. Quantitative real-time PCR analysis of the expression profiles showed that the CsbHLH2 gene was expressed in bud, leaf, stem and root. The highest expression level of the CsbHLH2 was found in the young leaf. Different hormone treatments results showed that the CsbHLH2 was induced by ETH, MEJA and SA treatment, respectively.

Key words

Camellia sinensis (L.) / bHLH transcription factor / expression analysis / phylogenetic analysis / subcelleular locatlization

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HAN Yongtao, XIAO Bin, QIAN Wenjun, LIANG Shaoru. Cloning and Expression Analysis of CsbHLH2 in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science. 2015, 35(5): 481-490 https://doi.org/10.13305/j.cnki.jts.2015.05.012

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