Study on the Protective Effect of L-Theanine on the Intestinal in Mice with Immune Stress Induced by ETEC

LIU Zunying; LIU Qiuling; DENG Yanli; CHEN Ling; XIAO Wenjun

doi:10.13305/j.cnki.jts.2016.05.004

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Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (5) : 469-476. DOI: 10.13305/j.cnki.jts.2016.05.004

Study on the Protective Effect of L-Theanine on the Intestinal in Mice with Immune Stress Induced by ETEC

LIU Zunying^1,2, LIU Qiuling^1,2, DENG Yanli^1,2, CHEN Ling^1,2, XIAO Wenjun^1,2,3,*

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Abstract

SPF grade Balb/c female mice were used as experimental animals. After adaptive feeding for 3 days, mice were orally pre-treated with different dosages of L-theanine once daily for 30 days before ETEC E44813 intraperitoneal injection. The body weight change in mice was analyzed during the breeding process. Mice were scarified 5 hours after ETEC injection and tissues were collected for analysis. The activities of ileum Glutathione peroxidase (GSH-Px), homogenate Catalase (CAT), Malondialdehyde (MDA) and Inducible nitric oxide synthase (iNOS) were measured and the pathological changes of intestinal and the levels of serum Tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β) and Intercellular cell adhesion molecule-1(ICAM-1) were examined. This study was

Key words

Enterotoxigenic Escherichia coli. / immune stress / intestinal protection / L-theanine

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LIU Zunying, LIU Qiuling, DENG Yanli, CHEN Ling, XIAO Wenjun. Study on the Protective Effect of L-Theanine on the Intestinal in Mice with Immune Stress Induced by ETEC[J]. Journal of Tea Science. 2016, 36(5): 469-476 https://doi.org/10.13305/j.cnki.jts.2016.05.004

References

[1] Sonnenburg J L, Angenent L T, Gordon J I.Getting a grip on things: how do communities of bacterial symbionts become established in our intestine[J]. Nature Immunology, 2004, 5(6): 569-573.
[2] 王其洲, 周元军, 黄胜昌, 等. 人兽共患病及其防治[J]. 医学动物防制, 2005, 21(9): 675-676.
[3] Qadri F, Svennerholm A M, Faruque A S G, et al. Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention[J]. Clinical microbiology reviews, 2005, 18(3): 465-483.
[4] GiIIigan P H. Escherichia coIi EAEC, EHEC, EIEC, ETEC[J]. Clinics in laboratory medicine, 1999, 19(3): 505-521.
[5] 王星, 覃宗华, 刘定发. 大肠杆菌不耐热肠毒素的分子生物学及黏膜免疫佐剂效应[J]. 广东畜牧兽医科技, 2005, 30(4): 18-21.
[6] 李成洪, 王孝友, 杨睿. 植物提取物饲料添加剂对生长猪生产性能的影响[J]. 饲料工业, 2012, 33(17): 14-16.
[7] 卫智权, 阎莉, 邓家刚. 芒果苷对脂多糖诱导的慢性炎症大鼠MAPK通路及血清细胞因子的影响[J]. 中草药, 2013, 44(1): 52-58.
[8] 宋丹, 张立艳, 白换力. 香芪汤及其有效成分对LPS诱导的巨噬细胞MAPK信号通路的影响[J]. 中国兽医学报, 2013, 33(5): 776-779.
[9] Juneja L R, Chu D C, Okubo T, et al.L-theanine-a unique amino acid of green tea and its relaxation effect in humans[J]. Trends in Food Science & Technology, 1999, 10(6): 199-204.
[10] Kitaoka S, Hayashi H, Yokogoshi H, et al.Transmural potential changes associated with the in vitro absorption of theanine in the guinea pig intestine[J]. Bioscience, Biotechnology and Biochemistry, 1996, 60(11): 1768-1771.
[11] Nishida K, YasudA E, Nagasawa K, et al. Altered levels of oxidation and phospholipase C isozyme expression in the brains of theanine -administered rats[J]. Biological & Pharmaceutical Bulletin, 2008, 31(5): 857-860.
[12] 文慧, 魏时来, 张石蕊, 等. L-茶氨酸对黄羽肉鸡生产性能和免疫功能的影响[J]. 动物营养学报, 2012, 24(10): 1946-1954.
[13] 孙浪, 鲁双庆, 肖调义, 等. 饲料中添加茶氨酸对草鱼免疫和血清生化指标的影响[J]. 饲料研究, 2012(6): 63-65.
[14] 谭俊峰, 林智, 李靓. 茶氨酸复合制剂增强免疫力的功能研究[J]. 茶叶科学, 2012, 32(3): 224-228.
[15] 王庆利, 张力军, 吴明春. 茶氨酸对脑缺血损伤大鼠自由基代谢的影响[J]. 实用医学杂志, 2008, 24(11): 1898-1900.
[16] 刘婉如. 茶氨酸神经保护作用的体外和体内实验研究[D]. 北京: 中国疾病预防控制中心, 2007: 14-62.
[17] 张燕辉, 吴明春, 王庆利. 茶氨酸对大鼠局灶性脑缺血性损伤后炎性细胞因子表达的影响[J]. 中国临床神经外科杂志, 2010, 15(9): 544-546.
[18] De Roux N, Genin E, Carel J C, et al.Hypogonadotropic hypogonadism due to loss of function of the KiSSl-deftved peptide receptor GPR54[J]. Proceedings of the National Academy of Sciences, 2003, 100(19): 10972-10976.
[19] Borzelleca JF, Peters D, Hall W.A 13-week dietary toxicity and toxicokinetic study with L-theanine in rats[J]. Food and Chemical Toxicology, 2006, 44(7): 1158-1166.
[20] Inoue T, Tsuji T, Koto M, et al.Amino acid sequence of heat-labile enterotoxin from chicken enterotoxigenic Escherichia coli is identical to that of human strain H 10407[J]. FEMS Microbiology Letters, 1993, 108(2): 157-162.
[21] 陈培富, 朱兴全. 大肠杆菌耐热肠毒素的结构与特性[J]. 中国预防兽医学报, 2012, 34(3): 244-246.