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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (4): 537-548.doi: 10.13305/j.cnki.jts.20220416.004

• Research Paper • Previous Articles     Next Articles

Residue Determination of Sixteen Pesticides in Green Tea by UPLC-MS/MS

LI Ziqiang1,2, YANG Mei1,*, ZHANG Xinzhong1,*, LUO Fengjian1, ZHOU Li1, LOU Zhengyun1, SUN Hezhi1, WANG Xinru1, CHEN Zongmao1   

  1. 1. Research Center of Quality Safety for Agricultural Products, Tea Research Institute, Chinese Academy of Agricultural Sciences Hangzhou 310008, China;
    2. College of Plant Protection, Jilin Agricultural University, Changchun 130000, China
  • Received:2021-11-02 Revised:2022-02-17 Online:2022-08-15 Published:2022-08-23

Abstract: An analytical method was developed for the simultaneous determination of 16 pesticide residues in green tea by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The target pesticides in tea samples were extracted by acetonitrile with 1% formic acid, cleaned up by a TPT-SPE column, ionized in electrospray positive ionization source (ESI+) mode, and scanned for determination of the target parent and daughter ions by mass spectrometry in multiple reaction monitoring mode (MRM). The results show that the target pesticides were linear in the range of 0.005-1.000 mg·kg-1 with the correlation coefficient R2>0.992 6. The average recoveries ranged from 74.0% to 105.4% at the four spiked levels of 0.010, 0.050, 0.100、1.000 mg·kg-1, and the relative standard deviations (RSD) were <20.0%. The limits of quantification were 10-50 μg·kg-1. The method is fast and sensitive, and the technical parameters are in accordance with the relevant domestic and international regulations and standards. And it can meet the requirements of simultaneous determination of various pesticide residues in green tea.

Key words: multi-residue of pesticides analysis, UPLC-MS/MS, TPT-SPE column clean, green tea

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