Analysis of Genetic Diversity and Genetic Structure in Geographic Populations of Stephanitis chinensis from China Based on Mitochondrial DNA COI Sequence

CHEN Shichun; JIANG Hongyan; LIAO Shuran; CHEN Tingxu; WANG Xiaoqing

doi:10.13305/j.cnki.jts.2023.06.008

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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (6) : 795-805. DOI: 10.13305/j.cnki.jts.2023.06.008

Analysis of Genetic Diversity and Genetic Structure in Geographic Populations of Stephanitis chinensis from China Based on Mitochondrial DNA COI Sequence

CHEN Shichun, JIANG Hongyan, LIAO Shuran, CHEN Tingxu, WANG Xiaoqing^*

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Abstract

The tea lace bug, Stephanitis chinensis, is an important pest of the southwest tea region in China, which has spread and caused disasters in recent years. To analyze the ecological adaptation mechanism and disaster law of S. chinensis, COI sequences of 240 adults from 12 populations of this pest were sequenced. The genetic differentiation, gene flow level and molecular variance were analyzed by DnaSP 6.12.03, Arlequin 3.5.2.2 and MEGA 7.0.26, respectively. There were 75 mutation sites and 38 haplotypes in the COI sequences of 12 geographic populations, and only Hap13 was a shared haplotype. Haplotype diversity index (H_d) of the total population was 0.827 79, H_d values between geographical populations ranged from 0.00 to 0.85. Fixed coefficient (F_ST) and gene flow (N_m) value of total population were 0.864 26 and 0.039 87, respectively. The results indicate that there are a high degree of genetic differentiation and a small degree of gene exchange of the total population in China. Population pairs of 5 populations (CQCK, CQWX, HBES, HBSY and SXHZ) had low genetic differentiation and frequent gene exchange (F_ST<0.06, N_m>4.50), while other population pairs had high genetic differentiation and less gene exchange (F_ST>0.25, N_m<1.00). Molecular variance analysis (AMOVA) supports that the genetic differentiation was mainly among populations (86.43%). Tajima's D and Fu's F_s neutrality test support that population expansion events occurred in the CQBN, HBES populations and the whole population around the Daba Mountains. In this study, the risk of both invasion expansion and original population expansion of S. chinensis in China was analyzed and speculated. It suggests that the field monitoring of the tea lace bug should be strengthened in tea plantations.

Key words

Stephanitis chinensis / COI gene / genetic diversity / genetic structure / geographic population

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CHEN Shichun, JIANG Hongyan, LIAO Shuran, CHEN Tingxu, WANG Xiaoqing. Analysis of Genetic Diversity and Genetic Structure in Geographic Populations of Stephanitis chinensis from China Based on Mitochondrial DNA COI Sequence[J]. Journal of Tea Science. 2023, 43(6): 795-805 https://doi.org/10.13305/j.cnki.jts.2023.06.008

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