Identification and Tissue Expression Analysis of Sucrose Transporter (SUT) Gene Family in Camellia sinensis

LUO Wei; ZHANG Jiaqi; YANG Ni; HU Zhihang; HAO Jiannan; LIU Hui; TAN Shanshan; ZHUANG Jing

doi:10.13305/j.cnki.jts.2024.04.002

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Journal of Tea Science ›› 2024, Vol. 44 ›› Issue (4) : 585-597. DOI: 10.13305/j.cnki.jts.2024.04.002

Research Paper

Identification and Tissue Expression Analysis of Sucrose Transporter (SUT) Gene Family in Camellia sinensis

LUO Wei¹, ZHANG Jiaqi², YANG Ni¹, HU Zhihang¹, HAO Jiannan¹, LIU Hui², TAN Shanshan², ZHUANG Jing^1,*

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Abstract

Sucrose transporters (SUTs), the main sucrose carriers, consume energy to transport and load sucrose, which play a key role in the transport of plant photosynthetic products from source to sink. In this study, seven members of CsSUTs family were identified from Camellia sinensis ‘Shuchazao’ by bioinformatics analysis. Their physical and chemical properties, gene structure, subcellular localization, evolutionary relationship and cis-acting elements were analyzed. The identified CsSUT proteins, containing a conserved MFS-2 domain, are closely related to AtSUC proteins in Arabidopsis thaliana, which are clustered in SUTⅠ, Ⅱ and Ⅳ. AtSUC proteins of Arabidopsis thaliana were used as a model in the STRING online website to speculate that there might be a direct interaction between CsSUT proteins and SWEET, SUS and STP proteins. Analysis of the promoter regions of the CsSUT family genes in tea plants reveals that there were masses of cis-acting elements related to hormone response, abiotic stress, and plant growth and development. It is speculated that these promoters may be regulated by plant hormones, stress and other factors, thus affecting the growth and development of tea plants. There were differences in the expression patterns of CsSUT family genes in C. sinensis ‘Longjing 43’ and C. sinensis ‘Shuchazao’. CsSUT6 was highly expressed in flowers, suggesting that it may contribute to the supply, storage and distribution of sucrose in floral organs. CsSUT1 and CsSUT5 were highly expressed in various organs of tea plants, indicating that they may synergistically participate in the process of sucrose loading in ‘ source’ leaves and unloading in ‘sink’ organs.

Key words

Camellia sinensis / bioinformatics analysis / promoter analysis / sucrose transporters / tissue specificity

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LUO Wei, ZHANG Jiaqi, YANG Ni, HU Zhihang, HAO Jiannan, LIU Hui, TAN Shanshan, ZHUANG Jing. Identification and Tissue Expression Analysis of Sucrose Transporter (SUT) Gene Family in Camellia sinensis[J]. Journal of Tea Science. 2024, 44(4): 585-597 https://doi.org/10.13305/j.cnki.jts.2024.04.002

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