Isolation and Identification of Aspergillus cristatus LJSC.2006 and Its Effect on Fu Tea&#x02019;s Quality

XIAO Juanjuan; CHENG Ying; LIU Yan; LIU Qiaofang; JIANG Ating; HUANG Jian'an; WANG Kunbo; LIU Zhonghua; WANG Zhenhong; YU Lijun

doi:10.13305/j.cnki.jts.2024.04.009

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Journal of Tea Science ›› 2024, Vol. 44 ›› Issue (4) : 639-654. DOI: 10.13305/j.cnki.jts.2024.04.009

Research Paper

Isolation and Identification of Aspergillus cristatus LJSC.2006 and Its Effect on Fu Tea’s Quality

XIAO Juanjuan¹, CHENG Ying², LIU Yan³, LIU Qiaofang¹, JIANG Ating¹, HUANG Jian'an^1,4,5,6, WANG Kunbo^1,4,5,6, LIU Zhonghua^1,4,5,6, WANG Zhenhong^2,*, YU Lijun^1,4,5,6,*

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Abstract

This study investigated the effect of self-isolated and purified strain LJSC.2006 on the quality of Hunan Fu tea, a primary dark tea. Strain LJSC.2006 was identified as Aspergillus cristatus (Aspergillus cristatus LJSC.2006, GenBank accession number: MZ147025) through colony plate morphology, spore electron microscopy, and mycelial molecular marker identification. Sensory evaluation, biochemical composition analysis and head space solid phase microextraction/gas chromatography-mass spectrometry methods were applied to assess the flavor and aroma qualities of dark tea raw materials and fermented Jinhua loose tea. The results indicate that compared with the primary dark tea, the fermented loose tea sample exhibited a deeper color, the golden flowers, a richer fungus aroma, and a mellower taste. After fermentation by Aspergillus cristatus LJSC.2006, there was a decrease in the levels of flavor quality components such as tea polyphenols, soluble carbohydrates, free amino acids, flavonoids, ester-catechins, myricetin, quercetin and kaempferol. The aroma components, esters and aldehydes of the loose tea samples increased after fermentation. Styrene and cedrol were the common aroma components in the primary dark tea. (E)-linalool 3,7-oxide and acetophenone were the common aroma components in the fermented loose tea. Additionally, ten characteristic volatile components relative content were significantly increased, including methyl salicylate, (E,E)-2,4-heptadienal, (E)-linalool-3,7-oxide, (E)-furan oxidized linalool, (E)-2-nonanal, (E)-2-hexenal, (E,Z)-6-nonanal, acetophenone, (E)-2-nonanal, and methyl vanillate, which together contributed to the distinctive fungal fragrance of Jinhua loose tea.

Key words

Aspergillus cristatus / fermented quality / fungi strain identification / Jinhua loose tea / primary dark tea

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XIAO Juanjuan, CHENG Ying, LIU Yan, LIU Qiaofang, JIANG Ating, HUANG Jian'an, WANG Kunbo, LIU Zhonghua, WANG Zhenhong, YU Lijun. Isolation and Identification of Aspergillus cristatus LJSC.2006 and Its Effect on Fu Tea’s Quality[J]. Journal of Tea Science. 2024, 44(4): 639-654 https://doi.org/10.13305/j.cnki.jts.2024.04.009

References

[1] 吕嘉枥, 杨柳青, 孟雁南. 茯砖茶中金花菌群的研究进展[J]. 食品科学, 2020, 41(9): 316-322.
Lü J L, Yang L Q, Meng Y N.Development of golden flower fungus community in Fuzhuan brick tea: a review[J]. Food Science, 2020, 41(9): 316-322.
[2] 沈程文, 邓岳朝, 周跃斌, 等. 湖南茯砖茶品质特征及其香气组分研究[J]. 茶叶科学, 2017, 37(1): 38-48.
Shen C W, Deng Y Z, Zhou Y B, et al.Research of quality features and aroma components in Hunan Fu brick tea[J]. Journal of Tea Science, 2017, 37(1): 38-48.
[3] Xiang X L, Su C, Shi Q X, et al.Potential hypoglycemic metabolites in dark tea fermented by Eurotium cristatum based on UPLC-QTOF-MS/MS combining global metabolomic and spectrum-effect relationship analyses[J]. Food Function, 2021, 12(16): 7546-7556.
[4] Xiao Y, Huang Y N, Long F W, et al.Insight into structural characteristics of theabrownin from Pingwu Fuzhuan brick tea and its hypolipidemic activity based on the in vivo zebrafish and in vitro lipid digestion and absorption models[J]. Food Chemistry, 2023, 404: 134382. doi: 10.1016/j.foodchem. 2022.134382.
[5] 代昕玥, 葛炳钢, 张旭雯, 等. 茯砖茶改善2型糖尿病小鼠代谢紊乱的效果研究[J]. 茶叶科学, 2022, 42(1): 63-75.
Dai X Y, Ge B G, Zhang X W, et al.Effect of Fu brick tea on improving metabolic disorders in type 2 diabetes mice[J]. Journal of Tea Science, 2022, 42(1): 63-75.
[6] Lu X J, Jing Y, Li Y Y, et al.The differential modulatory effects of Eurotium cristatum on the gut microbiota of obese dogs and mice are associated with improvements in metabolic disturbances[J]. Food Function, 2021, 12(24): 12812-12825.
[7] Sun Y J, Wang F, Liu Y, et al.Microbiome-metabolome responses of Fuzhuan brick tea crude polysaccharides with immune-protective benefit in cyclophosphamide-induced immunosuppressive mice[J]. Food Research International, 2022, 157: 111370. doi: 10.1016/j.foodres.2022.111370.
[8] Du Y, Yang W R, Yang C C, et al.A comprehensive review on microbiome, aromas and flavors, chemical composition, nutrition and future prospects of Fuzhuan brick tea[J]. Trends in Food Science & Technology, 2022, 119: 452-466.
[9] 赵仁亮, 吴丹, 姜依何, 等. 不同产区加工的茯砖茶中“金花”菌的分离及分子鉴定[J]. 湖南农业大学学报(自然科学版), 2016, 42(6): 592-600.
Zhao R L, Wu D, Jiang Y H, et al.Isolation and molecular identification of “Jinhua” fungi on the Fuzhuan tea produced in different regions[J]. Journal of Hunan Agricultural University (Natural Sciences), 2016, 42(6): 592-600.
[10] 齐祖同, 孙曾美. 茯砖茶中优势菌种的鉴定[J]. 真菌学报, 1990(3): 176-179.
Qi Z T, Sun Z M.Identification of predominant species in brick tea[J]. Mycosystema, 1990(3): 176-179.
[11] Hubka V, Kolařík M, Kubátová A, et al.Taxonomic revision of Eurotium and transfer of species to Aspergillus[J]. Mycologia, 2013, 105(4): 912-937.
[12] 吕嘉枥, 孟雁南, 史朝烨, 等. 陕西茯茶中“金花菌”的ITS序列特性分析[J]. 陕西科技大学学报, 2018, 36(4): 47-51.
Lü J L, Meng Y N, Shi Z Y, et al.Analysis of the characteristics of ITS sequences of “JinHua” fungus in Shaanxi Fuzhuan tea[J]. Journal of Shaanxi University of Science & Technology, 2018, 36(4): 47-51.
[13] 谭玉梅, 王亚萍, 葛永怡, 等. 贵州地区茯砖茶“金花菌”的分离和分子鉴定[J]. 菌物学报, 2017, 36(2): 154-163.
Tan Y M, Wang Y P, Ge Y Y, et al.Solation and molecular identification of Asperaillus cristatus in fermented “fuzhuan” brick tea from Guizhou Province[J]. Mycosystema, 2017, 36(2): 154-163.
[14] Xu S, Zhou Y F, Yu L J, et al.Protective effect of Eurotium cristatum fermented loose dark tea and Eurotium cristatum particle on MAPK and PXR/AhR signaling pathways induced by electronic cigarette exposure in mice[J]. Nutrients, 2022, 14(14): 2843. doi: 10.3390/nu14142843.
[15] White T J, Bruns T, Lee S, et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics [M]. San Diego: Academic Press, Inc.,1990: 315-322.
[16] Glass N L, Donaldson G C.Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes[J]. Applied and Environmental Microbiology, 1995, 61(4): 1323-1330.
[17] Peterson S W.Phylogenetic analysis of Aspergillus species using DNA sequences from four loci[J]. Mycologia, 2008, 100(2): 205-226.
[18] Liu Y J, Whelen S, Hall B D.Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse Ⅱ subunit[J]. Molecular Biology and Evolution, 1999, 16(12): 1799-1808.
[19] 史云峰, 禹利君, 胥锦桦, 等. 皱边石杉内生真菌DNA快速有效提取的研究[J]. 中国农学通报, 2012, 28(6): 183-188.
Shi Y F, Yu L J, Xu J H, et al.A rapid and effective method for isolating total DNA from Huperzia crispata's endophyte fungi mycelia[J]. Chinese Agricultural Science Bulletin, 2012, 28(6): 183-188.
[20] 钟萝. 茶叶品质理化分析[M]. 上海: 科学技术出版社, 1989: 400-402.
Zhong L.Physicochemical analysis of tea quality [M]. Shanghai: Science and Technology Press, 1989: 400-402.
[21] Samanidou V, Tsagiannidis A, Sarakatsianos I.Simultaneous determination of polyphenols and major purine alkaloids in Greek Sideritis species, herbal extracts, green tea, black tea, and coffee by high-performance liquid chromatography-diode array detection[J]. Journal of Separation Science, 2012, 35(4): 608-615.
[22] 李家华, 赵明, 张广辉, 等. 茶树新品种“紫娟”茶中杨梅素、槲皮素和山柰酚的HPLC分析[J]. 云南农业大学学报(自然科学), 2012, 27(2): 235-240.
Li J H, Zhao M, Zhang G H, et al.HPLC analysis of myricetin, quercetin and kaempferol in fresh shoots of “Zijuan” tea, a new cultivar of Camelia sinensis var. assamica[J]. Journal of Yunnan Agricultural University (Natural Science), 2012, 27(2): 235-240.
[23] 彭晓赟, 章卫民, 刘淑云, 等. 湖南地区茯砖茶中金花菌的分离鉴定[J]. 菌物研究, 2011, 9(3): 157-161.
Peng X Y, Zhang W M, Liu S Y, et al.Isolation and identification of “Golden-Flower” fungus in Fuzhuan tea from Hunan area[J]. Mycosystema, 2011, 9(3): 157-161.
[24] Rui Y, Wan P, Chen G J, et al.Analysis of bacterial and fungal communities by Illumina MiSeq platforms and characterization of Aspergillus cristatus in Fuzhuan brick tea[J]. LWT, 2019, 110: 168-174.
[25] Xiao Y, Li M Y, Liu Y, et al.The effect of Eurotium cristatum (MF800948) fermentation on the quality of autumn green tea[J]. Food Chemistry, 2021, 358: 129848. doi: 10.1016/j.foodchem.2021.129848.
[26] Ye J H, Ye Y, Yin J F, et al.Bitterness and astringency of tea leaves and products: formation mechanism and reducing strategies[J]. Trends in Food Science & Technology, 2022, 123: 130-143.
[27] Li H H, Luo L Y, Wang J, et al.Lexicon development and quantitative descriptive analysis of Hunan fuzhuan brick tea infusion[J]. Food Research International, 2019, 120: 275-284.
[28] Li Q, Li Y D, Luo Y, et al.Characterization of the key aroma compounds and microorganisms during the manufacturing process of Fu brick tea[J]. LWT, 2020, 127: 109355. doi: 10.1016/j.foodres.2019.02.047.
[29] Xiao Y, Huang Y X, Chen Y L, et al.Discrimination and characterization of the volatile profiles of five Fu brick teas from different manufacturing regions by using HS-SPME/GC-MS and HS-GC-IMS[J]. Current Research in Food Science, 2022, 5: 1788-1807.
[30] Zheng X X, Hu T F, Jin Y L, et al.Characterization of key aroma compounds and relationship between aroma compounds and sensory attributes in different aroma types of Fu brick tea[J]. Food Chemistry: X, 2022, 13: 100248. doi: 10.1016/j.fochx.2022.100248.
[31] Zhang W C, Cao X, Liu S Q.Aroma modulation of vegetable oils: a review[J]. Critical Reviews in Food Science and Nutrition, 2020, 60(9): 1538-1551.
[32] Frija L M T, Frade R F M, Afonso C A M. Isolation, chemical, and biotransformation routes of labdane-type diterpenes[J]. Chemical Reviews, 2011, 111(8): 4418-4452.
[33] 罗密, 俞梦瑶, 禹利君, 等. 冠突散囊菌LJSC.2001对不同黑毛茶发花品质的影响[J]. 食品科学, 2023, 44(14): 106-115.
Luo M, Yu M Y, Yu L J, et al.Effects of fermentation by Eurotium cristatum LJSC.2001 on the qualities of different primary dark tea[J]. Food Science, 2023, 44(14): 106-115.
[34] 汤依钰, 俞梦瑶, 禹利君, 等. 冠突散囊菌LJSC.2005对茯茶主体黑毛茶发花品质影响[J]. 茶叶科学, 2022, 42(6): 851-862.
Tang Y Y, Yu M Y, Yu L J, et al.Effects of Eurotium cristatum LJSC.2005 on the quality of primary dark tea, a major part of Fu tea[J]. Journal of Tea Science, 2022, 42(6): 851-862.