Isolation and Identification of the Pathogen Causing Leaf Spot in Tea Plants

YANG Fang; JIANG Bingbing; LEI Jinmei; GUO Cunwu; LI Limei; XU Jiayi; WANG Xinghua; YUAN Wenxia; WANG Baijuan

doi:10.13305/j.cnki.jts.2025.02.006

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Journal of Tea Science ›› 2025, Vol. 45 ›› Issue (2) : 266-272. DOI: 10.13305/j.cnki.jts.2025.02.006

Research Paper

Isolation and Identification of the Pathogen Causing Leaf Spot in Tea Plants

YANG Fang^1,2, JIANG Bingbing^1,2, LEI Jinmei^1,2, GUO Cunwu³, LI Limei^1,2, XU Jiayi^1,2, WANG Xinghua^1,2, YUAN Wenxia^1,2, WANG Baijuan^1,2,*

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Abstract

Tea plant [Camellia sinensis (L.) O. Kuntze] is an important agricultural economic crop in China. Tea leaf spot disease is one of the most important leaf diseases of tea plants. It seriously affects the yield and quality of tea. In 2023, a tea leaf spot disease was found in a tea garden in Menghai County, Xishuangbanna Dai Autonomous Prefecture Yunnan Province. In order to clarify the pathogen, the tissue isolation method and wound inoculation method were used to isolate and determine the pathogenicity of the pathogen. The morphological characteristics were observed and combined with molecular biology techniques to determine the type of pathogen. The results show that the three strains could infect tea leaves and were the causal agents of tea leaf spot. The pathogen causing tea leaf spot was identified as Nigrospora musae by observing its morphological characteristics and phylogenetic analysis of multiple genes (ITS, TUB2 and TEF-1α). The results of this study provided a theoretical basis for the prevention and control of tea leaf spot caused by the pathogen.

Key words

Camellia sinensis / Nigrospora musae / leaf spot disease / pathogen identification

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YANG Fang, JIANG Bingbing, LEI Jinmei, GUO Cunwu, LI Limei, XU Jiayi, WANG Xinghua, YUAN Wenxia, WANG Baijuan. Isolation and Identification of the Pathogen Causing Leaf Spot in Tea Plants[J]. Journal of Tea Science. 2025, 45(2): 266-272 https://doi.org/10.13305/j.cnki.jts.2025.02.006

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