Physiological Effects and Transcriptome Analysis of Different Concentrations of Indolebutyric Acid&#x000b7;S-Abscisic Acid on Tea Leaves Under Natural Low Temperature

LIANG Li; XIAO Huangqiao; SHI Yuling; LI Zhang; L&#x000dc; Xueying; JIE Yucheng

doi:10.13305/j.cnki.jts.2026.05.001

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Journal of Tea Science ›› 2026, Vol. 46 ›› Issue (5) : 831-842. DOI: 10.13305/j.cnki.jts.2026.05.001

Physiological Effects and Transcriptome Analysis of Different Concentrations of Indolebutyric Acid·S-Abscisic Acid on Tea Leaves Under Natural Low Temperature

LIANG Li^1,3, XIAO Huangqiao^1,3, SHI Yuling^1,3, LI Zhang^1,3, LÜ Xueying^1,3, JIE Yucheng^1,2,3,*

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Abstract

Regulating the cold resistance of tea plants is of great significance to the overwintering cultivation, stable yield and quality improvement of the tea industry. To explore the effect of plant growth regulators on the low temperature tolerance in tea plants, a tea cultivar ‘Baojing Huangjincha 1' was used as the experimental material in this study. Under the condition of natural low temperature, the leaves were sprayed with indole butyric acid·S-abscisic acid compound at concentration of 1 000 times (active ingredient 0.01 mg·L^-1, C1), 500 times (active ingredient 0.02 mg·L^-1, C2) and 250 times dilution (active ingredient 0.04 mg·L^-1, C3), with water sprayed onto the leaves as the control. The agronomic traits, photosynthetic parameters, relative chlorophyll content (SPAD value), main quality components (tea polyphenols and free amino acids) and stress-resistant physiological indexes (peroxidase activity and malondialdehyde content) of tea plants were systematically determined to comprehensively evaluate the physiological regulation effect of the compound on the cold tolerance of tea plants. RNA-seq analysis was further used for exploring its mechanism at a molecular level. The results show that spraying the compound agent improved leaf growth (length, width and thickness), significantly improved photosynthetic performance such as net photosynthetic rate and stomatal conductance (P<0.05), increased SPAD value, tea polyphenol and free amino acid contents, significantly enhanced POD activity and reduced MDA accumulation (P<0.05), and effectively alleviated membrane lipid peroxidation damage caused by low temperature. Principal component analysis shows that the best treatment was C2 (active ingredient 0.02 mg·L^-1). Transcriptome analysis shows that there were 1 150 differentially expressed genes in the C2 treatment compared with the control, of which 674 were up-regulated and 476 were down-regulated. KEGG enrichment analysis shows that the differential genes were mainly enriched in monoterpene biosynthesis, glutathione metabolism, linoleic acid metabolism, flavonoid biosynthesis, zeatin synthesis, carbon metabolism, riboflavin metabolism and secondary metabolite biosynthesis. This study reveals the mechanism of indolebutyric acid·S-abscisic acid to enhance the low temperature adaptability of tea plants by synergistically regulating photosynthetic characteristics, antioxidant system and secondary metabolic network at physiological and molecular levels, which provided a theoretical basis for the cultivation and management of cold-resistant tea plants and the breeding of cold-resistant cultivars.

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tea plant / natural low temperature / regulator / growth and development / transcriptome analysis

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LIANG Li, XIAO Huangqiao, SHI Yuling, LI Zhang, LÜ Xueying, JIE Yucheng. Physiological Effects and Transcriptome Analysis of Different Concentrations of Indolebutyric Acid·S-Abscisic Acid on Tea Leaves Under Natural Low Temperature[J]. Journal of Tea Science. 2026, 46(5): 831-842 https://doi.org/10.13305/j.cnki.jts.2026.05.001

References

[1] Hao X Y, Tang J W, Chen Y, et al. CsCBF1/CsZHD9-CsMADS27, a critical gene module controlling dormancy and bud break in tea plants[J]. The Plant Journal, 2025, 121(1): e17165. https://doi.org/10.1111/tpj.17165.
[2] Huang F F, Li Y, Yang P D, et al.Relationship between theanine, catechins and related genes reveals accumulation mechanism during spring and summer in tea plant (Camellia sinensis L.)[J]. Scientia Horticulturae, 2022, 302: 111142. https://doi.org/10.1016/j.scienta.2022.111142.
[3] Yao S H, Zhou C, Li S J, et al.Microbial diversity across tea varieties and ecological niches: correlating tea polyphenol contents with stress resistance[J]. Frontiers in Microbiology, 2024, 15: 1439630. https://doi.org/10.3389/fmicb.2024.1439630.
[4] Zhang X N, Wen B B, Zhang Y B, et al.Transcriptomic and biochemical analysis reveal differential regulatory mechanisms of photosynthetic pigment and characteristic secondary metabolites between high amino acids green-leaf and albino tea cultivars[J]. Scientia Horticulturae, 2022, 295: 110823. https://doi.org/10.1016/j.scienta.2021.110823.
[5] Yan F, Qu D, Chen X H, et al. Transcriptome analysis of 5-aminolevulinic acid contributing to cold tolerance in tea leaves (Camellia sinensis L.)[J]. Forests, 2023, 14(2): 198. https://doi.org/10.3390/f14020198.
[6] Ming C, Zhu X Z, Hou M Y, et al. Effects of low-temperature stress on cold resistance biochemical characteristics of Dali and Siqiu tea seedlings[J]. Horticulturae, 2024, 10(8): 823. https://doi.org/10.3390/horticulturae10080823.
[7] Wang P J, Jin S, Chen X J, et al. Chromatin accessibility and translational landscapes of tea plants under chilling stress[J]. Horticulture Research, 2021, 8(1): 96. https://doi.org/10.1038/s41438-021-00529-8.
[8] Gomasta J, Hassan J, Sultana H, et al.Interactive plant growth regulator and fertilizer application dataset on growth and yield attributes of tomato (Solanum lycopersicum L.)[J]. Data in Brief, 2024, 57: 111136. https://doi.org/10.1016/j.dib.2024.111136.
[9] Mushtaq T, Bano A, Ullah A.Effects of rhizospheric microbes, growth regulators, and biochar in modulating antioxidant machinery of plants under stress[J]. Journal of Plant Growth Regulation, 2024, 44(5): 1846-1867.
[10] Zaghloul E A M, Awad E S A, Mohamed I R, et al. Co-application of organic amendments and natural biostimulants on plants enhances wheat production and defense system under salt-alkali stress[J]. Scientific Reports, 2024, 14(1): 29742. https://doi.org/10.1038/s41598-024-77651-9.
[11] Makwana R J, Rathva V D, Panchal B H.Effect of IBA concentration (indol butaric acid) and growing condition on performance of cuttings in mogra (Jasminum sambac)[J]. Journal of Experimental Agriculture International, 2024, 46(10): 744-750.
[12] Xaxa S, Ilorkar V M, Toppo P, et al.Seasonal change and indole butyric acid (IBA) effect on survival and branch development in culm cuttings of Bambusa balcooa Roxb. and Dendrocalamus stocksii Munro[J]. Journal of Experimental Agriculture International, 2024, 46(11): 389-394.
[13] 刘昊, 宋晓波, 周乃富, 等. 吲哚丁酸对核桃嫩枝扦插生根及内源激素变化的影响[J]. 浙江农林大学学报, 2017, 34(6): 1038-1043.
Liu H, Song X B, Zhou N F, et al.Adventitious root formation with IBA and endogenous hormones dynamics in walnut soft-cuttings[J]. Journal of Zhejiang A & F University, 2017, 34(6): 1038-1043.
[14] Wang W X, Wang X X, Wang Y X, et al. S-ABA enhances rice salt tolerance by regulating Na+/K+ balance and hormone homeostasis[J]. Metabolites, 2024, 14(4): 181. https://doi.org/10.3390/metabo14040181.
[15] Yao C T, Zhang F W, Sun X, et al.Effects of S-abscisic acid (S-ABA) on seed germination, seedling growth, and Asr1 gene expression under drought stress in maize[J]. Journal of Plant Growth Regulation, 2019, 38(4): 1300-1313.
[16] Muñoz M D V M, Cabeza R L, Gámiz B, et al. Soil effects on the plant growth inhibitory activity of S-abscisic acid[J]. Biology and Fertility of Soils, 2024, 60(7): 955-968.
[17] MacDonald M T, Lada R R. Changes in endogenous hormone levels explains seasonal variation in balsam fir needle abscission patterns[J]. Journal of Plant Growth Regulation, 2017, 36(3): 723-733.
[18] 武建宽, 秦焕荣, 杨萍. S-诱抗素对小麦生长调节作用及增产效果试验研究[J]. 陕西农业科学, 2014, 60(2): 6-7.
Wu J K, Qin H R, Yang P.Experiment on the growth regulation and yield-increasing effect of S-abenomic acid on wheat[J]. Shaanxi Journal of Agricultural Sciences, 2014, 60(2): 6-7.
[19] 冒宇翔, 沈俊明, 陈惠, 等. 1% S-诱抗素·吲哚丁酸在水稻生产上的应用研究[J]. 现代农药, 2009, 8(3): 55-56.
Mao Y X, Shen J M, Chen H, et al.Application of 1% (+)-abscisic acid+4-indol-3-yl-butyric acid on the rice production[J]. Modern Agrochemicals, 2009, 8(3): 55-56.
[20] 何忠全, 肖亮, 毛建辉, 等. 脱落酸·吲哚丁酸在水稻上的应用研究[J]. 西南农业学报, 2008, 21(3): 597-601.
He Z Q, Xiao L, Mao J H, et al.Application of the mixture of abscisic acid and 3-indole butyric acid on rice[J]. Southwest China Journal of Agricultural Sciences, 2008, 21(3): 597-601.
[21] 肖黄巧, 周红灿, 揭雨成. 不同植物生长调节剂对茶叶低温胁迫的光合特性研究[J]. 中国农学通报, 2024, 40(2): 25-33.
Xiao H Q, Zhou H C, Jie Y C.Effects of different plant growth regulators on photosynthetic characteristics of tea at low temperature stress[J]. Chinese Agricultural Science Bulletin, 2024, 40(2): 25-33.
[22] 汪安然, 林开勤, 鄢东海, 等. 吲哚丁酸对‘贵绿1号'茶树扦插生根的影响研究[J]. 贵茶, 2023(4): 34-39.
Wang A R, Lin K Q, Yan D H, et al.Effect of indolebutyric acid on the rooting of tea plant variety ‘Guilv1'[J]. Journal of Guizhou Tea, 2023(4): 34-39.
[23] 张莹, 盛忠雷, 邓敏, 等. 施加外源IBA对南川大茶树扦插生根和茶树生长素应答因子(CsARFs)mRNA表达的影响[J]. 云南农业大学学报(自然科学), 2020, 35(4): 667-672, 681.
Zhang Y, Sheng Z L, Deng M, et al.Effect of applying indole-3-butyric acid on the rooting of Camellia sinensis cuttings and the mRNA expression of auxin response factor (CsARFs)[J]. Journal of Yunnan Agricultural University (Natural Science), 2020, 35(4): 667-672, 681.
[24] 张楠, 洪永聪, 王玉, 等. 脱落酸和水杨酸对越冬期茶树叶片抗寒生理指标的影响[J]. 北方园艺, 2010(22): 21-24.
Zhang N, Hong Y C, Wang Y, et al.Effects of abscisic acid and salicylic acid on several physiological indexes related to cold resistance of tea leaf during wintering period[J]. Northern Horticulture, 2010(22): 21-24.
[25] 马林龙, 卢素芳, 金孝芳, 等. 4种植物生长调节剂对茶树叶芽生长及茶叶品质的影响[J]. 茶叶通讯, 2019, 46(1): 43-47.
Ma L L, Lu S F, Jin X F, et al.Effects of four kinds of plant growth regulators on the shoot growth and quality of Camellia sinensis[J]. Journal of Tea Communication, 2019, 46(1): 43-47.
[26] 肖文敏, 任志红, 吴焕焕, 等. 植物生长调节剂对春茶新梢生长及品质的影响[J]. 农学学报, 2024, 14(11): 7-12.
Xiao W M, Ren Z H, Wu H H, et al.Effects of plant growth regulators on the growth and quality of spring tea shoots[J]. Journal of Agriculture, 2024, 14(11): 7-12.
[27] 陈德西, 何忠全, 刘欢, 等. 植物源调节剂碧护组合技术对提升茶叶产量的效果研究[J]. 资源开发与市场, 2017, 33(3): 360-363.
Chen D X, He Z Q, Liu H, et al.Study on effects of different vitacat combinations on yield of tea[J]. Resource Development & Market, 2017, 33(3): 360-363.
[28] Li N H, Pu K G, Zhang M, et al.Glycine betaine alleviates low temperature combined with low light stress in pepper (Capsicum annuum L.) by improving photosynthetic ability and regulating genes related m⁶A methylases[J]. Scientia Horticulturae, 2024, 338: 113767. https://doi.org/10.1016/j.scienta.2024.113767.
[29] Altaf M A, Shu H Y, Hao Y Y, et al. Melatonin affects the photosynthetic performance of pepper (Capsicum annuum L.) seedlings under cold stress[J]. Antioxidants, 2022, 11(12): 2414. https://doi.org/10.3390/antiox11122414.
[30] Zhang X H, Zhang L, Sun Y P, et al.Hydrogen peroxide is involved in strigolactone induced low temperature stress tolerance in rape seedlings (Brassica rapa L.)[J]. Plant Physiology and Biochemistry, 2020, 157: 402-415. https://doi.org/10.1016/j.plaphy.2020.11.006.
[31] Bajwa V S, Shukla M R, Sherif S M, et al.Role of melatonin in alleviating cold stress in Arabidopsis thaliana[J]. Journal of Pineal Research, 2014, 56(3): 238-245.
[32] Cao S F, Song C B, Shao J R, et al.Exogenous melatonin treatment increases chilling tolerance and induces defense response in harvested peach fruit during cold storage[J]. Journal of Agricultural and Food Chemistry, 2016, 64(25): 5215-5222.
[33] Chang J J, Guo Y L, Zhang Z X, et al. CBF-responsive pathway and phytohormones are involved in melatonin-improved photosynthesis and redox homeostasis under aerial cold stress in watermelon[J]. Acta Physiologiae Plantarum, 2020, 42: 159. https://doi.org/10.1007/s11738-020-03147-4.
[34] Zhang Q, Li D M, Wang Q, et al. Exogenous salicylic acid improves chilling tolerance in maize seedlings by improving plant growth and physiological characteristics[J]. Agronomy, 2021, 11(7): 1341. https://doi.org/10.3390/agronomy11071341.
[35] Natalia R, Anna I, Ekaterina H, et al. Exogenous methyl jasmonate improves cold tolerance with parallel induction of two cold-regulated (COR) genes expression in Triticum aestivum L.[J]. Plants, 2021, 10(7): 1421. https://doi.org/10.3390/plants10071421.
[36] Roy P C, Ojha D K, Sahu W, et al.Overexpression, purification and characterization of a heat-stable peroxisomal glutathione reductase from Oryza sativa involved in multifactorial abiotic stress tolerance[J]. Journal of King Saud University-Science, 2024, 36(11): 103575. https://doi.org/10.1016/j.jksus.2024.103575.
[37] Chen W Q, Miao Y L, Ayyaz A, et al.Anthocyanin accumulation enhances drought tolerance in purple-leaf Brassica napus: transcriptomic, metabolomic, and physiological evidence[J]. Industrial Crops & Products, 2025, 223: 120149. https://doi.org/10.1016/j.indcrop.2024.120149.
[38] Zhao P S, Xia Y, Qian C Z, et al. Flavonoid synthesis pathway response to low-temperature stress in a desert medicinal plant, Agriophyllum Squarrosum (Sandrice)[J]. Genes, 2024, 15(9): 1228. https://doi.org/10.3390/genes15091228.
[39] Bundit K, Suriyan C U, Bualuang F, et al.Paclobutrazol mitigates salt stress in indica rice seedlings by enhancing glutathione metabolism and glyoxalase system[J]. Biologia, 2018, 73(12): 1267-1276.