酶法修饰绿茶多糖对免疫低下模型小鼠免疫活性的影响

Abstract

Abstract: Green tea polysaccharides was modified by using glycosidase extracted from tea, the effect of enzymatic modification of green tea polysaccharides on immune function of the immunosuppressant mice inhibited by cyclophosphamide was studied. The results showed that, TPS and ETPS were positive in the cell immune function, humor immune function, mononuclear macrophage function and NK cell activity tests. So it can be concluded that they could obviously improve both the cell immune function and humor immune function in immunosuppressant mice. The results also made clear that the enzymatic modification could significantly enhance the immune activity of green tea polysaccharides. With the same dose, ETPS could improve the spleen index, the phagocytosis rate and index of peritoneal macrophage, the level of ear swell in DTH, HC50 in serum and NK cell activity compared with the original TPS. Collectively, these data proved that the immune activity of green tea polysaccharides after enzymatic modification was significantly improved.

Key words: green tea, tea polysaccharide, enzymatic modification, immunocompetence

CLC Number:

TS272
R285.5

YU Zhi, SHI Yu-tao, NI De-jiang. Effect of Enzymatic Modification Green Tea Polysaccharide on Immune Function of the Immunosuppressant Mice[J]. Journal of Tea Science, 2010, 30(S1): 567-572.

References 15

[1]	江用文, 程启坤. 中国茶叶年鉴2008版[M]. 北京: 中国农业出版社, 2009.
[2]	倪德江, 陈玉琼, 谢笔钧, 等. 绿茶、乌龙茶、红茶的茶多糖组成、抗氧化及降血糖作用研究[J]. 营养学报, 2004, 26(1): 57-60.
[3]	丁仁凤, 何普明. 茶多糖和茶多酚的降血糖作用研究[J]. 茶叶科学, 2005, 25(3): 219-224.
[4]	王元凤, 金征宇. 茶叶中多糖的分离及降血糖活性的研究[J]. 中草药, 2005, 36(10): 1453-1457.
[5]	陈海霞. 高活性茶多糖的一级结构表征、空间构象及生物活性的研究[D]. 武汉: 华中农业大学, 2002.
[6]	徐仲溪, 王坤波. 茶多糖化学及生物活性的研究[J]. 茶叶科学, 2004, 24(2): 75-81.
[7]	罗祖友, 陈根洪, 陈业, 等. 茶多糖抗肿瘤及免疫调节作用的研究[J], 食品科学, 2007, 28(8): 457-461.
[8]	王元凤, 金征宇. 茶叶中多糖的分离及降血糖活性的研究[J]. 中草药, 2005, 36(10): 1453-1457.
[9]	余志, 黄海波, 张芸, 等. 茶叶中β-D-半乳糖苷酶活性分析条件的优化及其在不同季节、嫩度和品种间的差异分析[J]. 食品科学, 2007, 28(10): 384-388.
[10]	黄雨三. 保健食品检验与评价技术规范实施手册: 2003版 [M]. 北京: 清华同方电子出版社, 2003.
[11]	梁进, 张剑韵, 崔莹莹, 等. 茶多糖的化学修饰及体外抗凝血作用研究[J]. 茶叶科学, 2008, 28(3): 166-171.
[12]	王元凤, 金征宇, 魏新林. 茶多糖金属络合物的制备及清除自由基活性研究[J]. 天然产物研究与开发, 2009, 21(3): 382-387.
[13]	Chen H X, Zhang M, Xie B J.Quantification of uronic acids in tea polysaccharide conjugates and their antioxidant properties[J]. J Agric Food Chem, 2004, 52(11): 3333-3336.
[14]	Chen H X, Zhang M, Qu Z S, et al. Compositional analysis and preliminary toxocological evaluation of a tea polysaccharide conjugate[J]. J Agric Food Chem, 2007, 55(6): 2256-2260.
[15]	王丁刚. 茶叶多糖的降血糖、抗炎及碳廓清作用[J]. 茶叶科学, 1991, 11(2): 173-174.

Effect of Enzymatic Modification Green Tea Polysaccharide on Immune Function of the Immunosuppressant Mice

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