Study on Expression Conditions of EGCG-O-Methyltransferase in Recombinant Escherichia coli Bacteria

FEI Dong-mei; LIN Zhi; LU Hai-peng; ZHANG Yue; TAN Jun-feng; GUO Li

doi:10.13305/j.cnki.jts.2011.04.001

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Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (4) : 333-340. DOI: 10.13305/j.cnki.jts.2011.04.001

Study on Expression Conditions of EGCG-O-Methyltransferase in Recombinant Escherichia coli Bacteria

FEI Dong-mei^1,2, LIN Zhi^1*, LU Hai-peng¹, ZHANG Yue¹, TAN Jun-feng¹, GUO Li¹

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Abstract

EGCG3"Me could be produced from EGCG catalyzed by EGCG-O-Methyltransferase. Taken the yield of EGCG3"Me as main index, the present study focused on the producing conditions of EGCG-O-Methyltransferase induced by IPTG in recombinant E. coli bacteria. Results showed that the optimum producing conditions were as follows: the concentration of IPTG was 0.05mmol/L, the induction time was 20h, the initial pH of medium was 7.0 and the induction temperature was 20℃.

Key words

EGCG-O-Methyltransferase / expression conditions / recombinant Escherichia coli

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FEI Dong-mei, LIN Zhi, LU Hai-peng, ZHANG Yue, TAN Jun-feng, GUO Li. Study on Expression Conditions of EGCG-O-Methyltransferase in Recombinant Escherichia coli Bacteria[J]. Journal of Tea Science. 2011, 31(4): 333-340 https://doi.org/10.13305/j.cnki.jts.2011.04.001

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