六堡茶多糖理化性质、体外抗氧化及其对人脐静脉血管内皮细胞的保护作用

Abstract

Abstract: Tea Polysaccharides were extracted and purified from Liubao tea. Three fractions of polysaccharides namely LTPS-30, LTPS-50 and LTPS-70 were then obtained from Liubao Tea Polysaccharides (LTPS) by 30%, 50% and 70% ethanol respectively. The main chemical compounds and monosaccharide compositions of these LTPS were investigated. The in vitro antioxidant activities of LTPS were determined by ABTS and FRAP assays. The potentially protective effects of LTPS on human umbilical vascular endothelial cells (HUVEC) were investigated according to the Na₂S₂O₃-induced model. The results showed that there were two main acidic polysaccharides in LTPS-70, LTPS-50 and LTPS-30. The order of molecular weight and total sugar content was LTPS-70<LTPS-50<LTPS-30. While the order of protein, polyphenol contents and antioxidant abilities determined by ABTS and FRAP assays showed the opposite trends. LTPS-70 exhibited the protective effects on HUVEC, indicating it might be applied in medical sciences in the future.

Key words: Liubao tea, tea polysaccharides (TPS), antioxidant activity, HUVEC

CLC Number:

YING Le, PAN Yue, WANG Yuefei, XU Ping. Physicochemical Properties, in vitro Antioxidant Activities and Protective Effects of Liubao Tea Polysaccharides on HUVEC[J]. Journal of Tea Science, 2017, 37(1): 25-37.

References 23

[1]	谢明勇, 聂少平. 茶叶多糖的研究进展[J]. 食品与生物技术学报, 2006, 25(2): 107-114.
[2]	周斌星, 孔令波, 陈军贤. 普洱茶多糖的提取及降血糖的研究[J]. 中国农学通报, 2009, 25(15): 55-59.
[3]	徐仲溪, 王坤波. 茶多糖化学及生物活性的研究[J]. 茶叶科学, 2004, 24(2): 75-81.
[4]	陈小强, 叶阳, 成浩, 等. 六堡茶的理化分析研究[J]. 中国农学通报, 2008, 24(7): 77-80.
[5]	刘小玲, 李颖, 姜元欣, 等. 广西六堡茶主要特征成分分析[J]. 北京工商大学学报: 自然科学版, 2012, 30(1): 46-50.
[6]	吴平, 张宏, 樊春燕, 等. 《六堡茶》广西地方标准的术语和定义介绍——兼谈对《茶叶感官审评术语》标准修订的建议[J]. 广东茶业, 2010(1): 46-49.
[7]	杜冠华. 血管内皮细胞损伤机制及保护药物的研究[J]. 基础医学与临床, 2004, 24(3): 258-263.
[8]	Chen X Q, Wang Y F, Wu Y L, et al.Green tea polysaccharide-conjugates protect human umbilical vein endothelial cells against impairments triggered by high glucose[J]. International Journal of Biological Macromolecules, 2011, 49(1): 50-54.
[9]	王岳飞, 罗子华, 邬新荣. 普洱茶提取物抗氧化及其对Na2S2O3诱导[J]. 茶叶科学, 2010, 30(6): 475-481.
[10]	武冰峰, 杨娟, 谢红, 等. 党参多糖对神经干细胞硫代硫酸钠损伤的保护作用[J]. 时珍国医国药, 2008, 19(2): 280-281.
[11]	崔宏春, 余继忠, 黄海涛, 等. 茶多糖的提取及分离纯化研究进展[J]. 茶叶, 2011, 37(2): 67-71.
[12]	任小盈, 李静, 马存强, 等. 茶多糖的提取与分离纯化技术研究新进展[J]. 安徽农业科学, 2014, 42(23): 993-7995, 7999.
[13]	郑德勇, 杨江帆, 潘晓云, 等. 白茶多糖的提取与结构初探[J]. 茶叶科学, 2010, 30(S1): 551-555.
[14]	杨泱, 刘仲华, 黄建安. 茶多糖的提取、分离、纯化、组成研究概况[J]. 中国食物与营养, 2009(5): 47-49.
[15]	Lv Y, Yang X, Zhao Y, et al.Separation and quantification of component monosaccharides of the tea polysaccharides from Gynostemma pentaphyllum by HPLC with indirect UV detection[J]. Food Chemistry, 2009, 112(3): 742-746.
[16]	张丽萍. 苹果多糖的分离纯化及其自由基消除活性与红外光谱分析 [D]. 咸阳:西北农林科技大学, 2007: 20-23.
[17]	谢亮亮, 蔡为荣, 张虹, 等. 茶多糖的分离纯化及其抗凝血活性[J]. 食品与发酵工业, 2012, 38(9): 191-195.
[18]	陈浩. 普洱茶多糖降血糖及抗氧化作用研究[D]. 杭州: 浙江大学, 2013: 35-40.
[19]	张竞之, 陈利国, 贾会欣, 等. 黄芪多糖对高血压病血瘀证患者血清损伤的人脐静脉内皮细胞形态学、活性的影响[J]. 中国实验方剂学杂志, 2010(16): 170-173.
[20]	方积年, 丁侃. 天然药物-多糖的主要生物活性及分离纯化方法[J]. 中国天然药物, 2007, 5(5): 338-347.
[21]	Mao L, Shao S, Sun S, et al.Purification, physicochemical characterization, and bioactivities of polysaccharides from Puerh tea[J]. Journal of Food and Nutrition Research, 2014, 2(12): 1007-1014.
[22]	Wang Y, Yang Z, Wei X.Antioxidant activities potential of tea polysaccharide fractions obtained by ultra filtration[J]. International Journal of Biological Macromolecules, 2012, 50(3): 558-564.
[23]	Chen H, Qu Z, Fu L, et al.Physicochemical properties and antioxidant capacity of 3 polysaccharides from green tea, Oolong tea, and black tea[J]. Journal of Food Science, 2009, 74(6): C469-C474.

Physicochemical Properties, in vitro Antioxidant Activities and Protective Effects of Liubao Tea Polysaccharides on HUVEC

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 23

Related Articles 7

Metrics

Comments

Recommended 10

[1]	YANG Gaozhong, PENG Qunhua, ZHANG Yue, SHI Jiang, LIN Zhi, LÜ Haipeng. Effects of Anaerobic Treatment on Amino Acid Composition and Biological Activities of Different Type Teas [J]. Journal of Tea Science, 2022, 42(2): 222-232.
[2]	HU Xiexin, YI Youjin, BO Lianyang, LI Gaoyang, ZHOU Hongli, ZHOU Jinwei, XIA Bo. Research on Separation, Identification and Transformation Products Transformed Kudzu Root of ′Jinhua′ Fungus [J]. Journal of Tea Science, 2016, 36(3): 268-276.
[3]	REN Hongtao, ZHOU Bin, QIN Taifeng, XIA Kaiguo, ZHOU Hongjie. Study on Antioxidant Activity of Volatile Components from Pu-erh Tea [J]. Journal of Tea Science, 2014, 34(3): 213-220.
[4]	WANG Yue-fei, LUO Zi-hua, WU Xin-rong, CHEN Hong-bo, XU Ping, YANG Xian-qiang. Antioxidtive Activities of Pu-erh Tea Extracts and Their Protective Effect on HUVEC Damage Induced by Na₂S₂O₃ [J]. Journal of Tea Science, 2010, 30(6): 475-481.
[5]	CAI Zhuo, JIANG Cai-ying, ZHAO Jing, MO Li-jun, ZHANG Xian, CHEN Qing-quan. Determination of Scavenging Effect of Tea on Hydroxyl Radical by Flow Injection Chemiluminescence Method [J]. Journal of Tea Science, 2010, 30(3): 163-166.
[6]	QI Xiang-yang. Study on the Analysis and Antioxidant Activities of Different Extracts from (-)-Epigallocatechin Gallate Oxidation Products [J]. Journal of Tea Science, 2008, 28(6): 436-442.
[7]	LIU Li-xiang, LIANG Xing-fei, SUN Yi, Tanguy Laura, YE Hong, ZENG Xiao-xiong. Determination of Polyphenolic Content and Antioxidant Activity of Kudingcha Made from Ilex kudincha C. J. Tseng [J]. Journal of Tea Science, 2008, 28(4): 289-293.