Physicochemical Properties, in vitro Antioxidant Activities and Protective Effects of Liubao Tea Polysaccharides on HUVEC

YING Le; PAN Yue; WANG Yuefei; XU Ping

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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (1) : 25-37.

Physicochemical Properties, in vitro Antioxidant Activities and Protective Effects of Liubao Tea Polysaccharides on HUVEC

YING Le, PAN Yue, WANG Yuefei, XU Ping^*

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Abstract

Tea Polysaccharides were extracted and purified from Liubao tea. Three fractions of polysaccharides namely LTPS-30, LTPS-50 and LTPS-70 were then obtained from Liubao Tea Polysaccharides (LTPS) by 30%, 50% and 70% ethanol respectively. The main chemical compounds and monosaccharide compositions of these LTPS were investigated. The in vitro antioxidant activities of LTPS were determined by ABTS and FRAP assays. The potentially protective effects of LTPS on human umbilical vascular endothelial cells (HUVEC) were investigated according to the Na₂S₂O₃-induced model. The results showed that there were two main acidic polysaccharides in LTPS-70, LTPS-50 and LTPS-30. The order of molecular weight and total sugar content was LTPS-70

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antioxidant activity / HUVEC / Liubao tea / tea polysaccharides (TPS)

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YING Le, PAN Yue, WANG Yuefei, XU Ping. Physicochemical Properties, in vitro Antioxidant Activities and Protective Effects of Liubao Tea Polysaccharides on HUVEC[J]. Journal of Tea Science. 2017, 37(1): 25-37

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