Cloning and Expression Analysis of Geranylgeranyl Diphosphate Synthase Gene CsGGDPS in Tea Plant (Camellia sinensis)

YAO Xueqian; YUE Chuan; YANG Guoyi; CHEN Dan; ZHANG Dongtao; CHEN Guixin; YE Naixing

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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (1) : 86-96.

Cloning and Expression Analysis of Geranylgeranyl Diphosphate Synthase Gene CsGGDPS in Tea Plant (Camellia sinensis)

YAO Xueqian, YUE Chuan, YANG Guoyi, CHEN Dan, ZHANG Dongtao, CHEN Guixin^*, YE Naixing^*

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Abstract

A full-length cDNA sequence encoding geranylgeranyl diphosphate synthase (GGDPS) was isolated from transcriptome database of tea plant, cloned from C. sinensis cv. Tieguanyin and named as CsGGDPS. The cDNA length of CsGGDPS was 1β661βbp, with an open reading frame (ORF) of 1β137βbp and deduced protein of 378 amino acids. The protein was deduced to contain 5 conserved domains with 2 functional domains of Isoprenoid-Biosyn-C1 superfamily. The sequence analysis showed that CsGGDPS was highly conserved and had the closest genetic relationship with Panax notoginseng. CsGGDPS was an instability and hydrophilic protein, which was predicted to be located in chloroplast but with no transmembrane structure and signal peptide. There were 20 phosphorylation sites within the polypeptide chain. Alpha helix was predicted to be the major secondary structure of CsGGDPS. The three-dimension structure of CsGGDPS was highly similar to GGPPS11 from Arabidopsis thaliana. The quantitative real-time PCR showed that CsGGDPS expression was increased during the developmental process and increased with the age of tea leaves. Meanwhile, its expression was also enhanced during the Zuoqing procedure. CsGGDPS was ubiquitously expressed in the C. sinensis cv. Huangdan, cv. Tieguanyin and their first filial generation cv. Jinguanyin, but with different expression levels.

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gene expression / GGDPS / tea aroma / Camellia sinensis

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YAO Xueqian, YUE Chuan, YANG Guoyi, CHEN Dan, ZHANG Dongtao, CHEN Guixin, YE Naixing. Cloning and Expression Analysis of Geranylgeranyl Diphosphate Synthase Gene CsGGDPS in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science. 2017, 37(1): 86-96

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