Digital Gene Expression Analysis of Tea Flowers without Pistil at Three Development Stages

LI Mei; CHEN Linbo; TIAN Yiping; XIA Lifei; SONG Weixi; LIANG Mingzhi; JIANG Changjun

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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (1) : 97-107.

Digital Gene Expression Analysis of Tea Flowers without Pistil at Three Development Stages

LI Mei¹, CHEN Linbo¹, TIAN Yiping¹, XIA Lifei¹, SONG Weixi¹, LIANG Mingzhi¹, JIANG Changjun^2,*

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Abstract

The expression patterns of important genes in tea flowers without pistil at the flower bud, alabastrum and florescence stages was studied by the transcriptome and digital gene expression profiling technology. The results found that genes associating with biosynthesis and metabolic activities were highly expressed in tea flowers during the flowering process. Six genes involved in auxin signal transduction and the A、C and E genes of ABCDE model might be closely correlated with the stamen development and response to pistil deletion, which were under complex regulation. The down- regulation of WUS2 and WUS8 in WUS family might also regulate the C and E genes, and result in the deletion of pistil. In the KNOX gene family, none homologous gene of KNOXⅡ was detected. The down-regulation of 3 homologous genes of the KNOX I might weaken the initiation of carpels and the growth of marginal tissues. According to the digital gene expression analysis, a preliminary understanding of the network associating with the pistil deletion and stamen development in tea flower was obtained, which provides a theoretical basis for further research on pistil deletion, stamen development, molecular mechanism of sterility and sex determination in tea plant.

Key words

digital gene expression profiling / floral organ regulation / pistil deletion / tea flowers / transcriptome

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LI Mei, CHEN Linbo, TIAN Yiping, XIA Lifei, SONG Weixi, LIANG Mingzhi, JIANG Changjun. Digital Gene Expression Analysis of Tea Flowers without Pistil at Three Development Stages[J]. Journal of Tea Science. 2017, 37(1): 97-107

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