SNP Detection and Mapping of Theanine Synthetase Gene in Tea Plant

LI Xiaojie; MA Jianqiang; YAO Mingzhe; CHEN Liang

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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (3) : 251-257.

SNP Detection and Mapping of Theanine Synthetase Gene in Tea Plant

LI Xiaojie, MA Jianqiang, YAO Mingzhe^*, CHEN Liang^*

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Abstract

The theanine synthetase (TS) gene is considered as the key functional gene for the synthesis of theanine in tea plant. In this study, an F₁ segregating population and its parents were used to detect single nucleotide polymorphism (SNP) in the TS gene for genetic mapping. According to the sequence alignment between the parents, three SNPs were found, and SNP735 was identified to be heterozygous in YS. SNP735 was subsequently transformed into dCAPS marker and used for genotyping in the F₁ population. The results showed that the segregation ratio of alleles at this SNP locus was close to 1:1. The dCAPS marker was mapped to LG03 at a position between TM299 and TM517 in the tea genetic map. Meanwhile, significant correlations between the dCAPS marker and the content of theanine and total amino acid were identified in this study.

Key words

TS / dCAPS marker / mapping / SNP / tea plant / theanine

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LI Xiaojie, MA Jianqiang, YAO Mingzhe, CHEN Liang. SNP Detection and Mapping of Theanine Synthetase Gene in Tea Plant[J]. Journal of Tea Science. 2017, 37(3): 251-257

References

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