Research on the Protective Effects of TFDG on IL-1&#x003b2;-induced Inflammatory Injury in Rat Chondrocytes in Vitro

ZHOU Ying; HUANG Qian; ZHANG Tian; CHEN Ping

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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (3) : 290-298.

Research on the Protective Effects of TFDG on IL-1β-induced Inflammatory Injury in Rat Chondrocytes in Vitro

ZHOU Ying¹, HUANG Qian², ZHANG Tian¹, CHEN Ping^1,*

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Abstract

The chondrocytes were isolated and harvested from knee articular surface of male Sprague-Dawley rats, which were identified by toluidine blue and type II collagen immunofluorescence staining. To investigate potential protective effects of theaflavin-3,3′-digallate (TFDG) on IL-1β-induced inflammatory injury in rat chondrocytes in vitro, a model of osteoarthritis was established through stimulating rat chondrocytes with IL-1β. Inverted phase contrast microscopy showed TFDG treatment significantly improved osteoarthritis chondrocytes morphology. Real-time PCR results showed that TFDG treatment not only up-regulated chondrocyte marker Col II mRNA expression, but also down-regulated pro-inflammatory cytokines IL-1β and IL-6 mRNA expression. ELISA analysis further confirmed TFDG treatment significantly decreased the secretion of inflammatory factors. Western blot results showed that TFDG treatment significantly inhibited the inflammation-related enzyme COX-2 expression. Taken together, these results indicate that TFDG has a preventive effect on IL-1β-induced inflammatory injury in rat chondrocytes in vitro by reducing inflammatory reaction.

Key words

chondrocytes / inflammation / osteoarthritis / TFDG

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ZHOU Ying, HUANG Qian, ZHANG Tian, CHEN Ping. Research on the Protective Effects of TFDG on IL-1β-induced Inflammatory Injury in Rat Chondrocytes in Vitro[J]. Journal of Tea Science. 2017, 37(3): 290-298

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