The Repellency and Fumigant Activity of Essential Oil from Invasive Plant (Ageratum conyzoides) against Leafhopper (Empoasca onukii)

WANG Qizhi; LIU Yumei; LI Shuming

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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (5) : 442-448.

The Repellency and Fumigant Activity of Essential Oil from Invasive Plant (Ageratum conyzoides) against Leafhopper (Empoasca onukii)

WANG Qizhi¹, LIU Yumei², LI Shuming¹

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Abstract

The biological activity of essential oil from the invasive plant species Ageratum conyzoides L. to leafhopper Empoasca onukii Matsuda was reported in this study. The effects of essential oil concentrations on E. onukii were also investigated by selecting different plants and using Y-tube olfactometer and modified fumigation methods. The chemical composition of essential oil was examined using gas chromatography-mass spectrometry (GC-MS) and their relative activities was also analyzed. The results indicated that essential oil concentrations from A. conyzoides had significant effects on fumigant and repellent properties against E. onukii. High concentrations of essential oil displayed significant powerful effects. At an oil concentration of 200βμL·mL^-1, the repellent rate was 100%. At concentrations of 100βμL·mL^-1 and 50βμL·mL^-1, the repellent rates were 91.67%±1.67% and 76.67%±1.67%, respectively. At a concentration of 5βμL·mL^-1, the fumigant mortality rate at 12βh was 100%. Twenty-four essential oil constituents were identified, with the major constituents being β-caryophyllene (61.53%), precocene I (11.09%), and precocene II (7.79%). Main synergistic composition could effectively increase the repellency and fumigant activity against E. onukii. The results showed that the essential oil of A. conyzoides had significant effects against E. onukii and potential benefits as a botanical pesticide to control E. onukii.

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Empoasca onukii / Ageratum conyzoides / biological activity / chemical components

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WANG Qizhi, LIU Yumei, LI Shuming. The Repellency and Fumigant Activity of Essential Oil from Invasive Plant (Ageratum conyzoides) against Leafhopper (Empoasca onukii)[J]. Journal of Tea Science. 2017, 37(5): 442-448

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