RNA Sequencing Analysis of Fungi Community Diversity in Mildew Raw Dark Tea

XU Wei; JIANG Yihe; WU Dan; ZHAO Renliang; ZHU Qi

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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (5) : 483-492.

RNA Sequencing Analysis of Fungi Community Diversity in Mildew Raw Dark Tea

XU Wei, JIANG Yihe, WU Dan, ZHAO Renliang, ZHU Qi^*

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Abstract

In order to understand the feature of microbe in the mildew dark tea under high humidity conditions, Illumina Miseq high-throughput sequencing was performed to study fungi diversity by artificial culture of certain fungi. The influence of the OTU (Operational Taxonomic Unit) threshold on the bioinformatic analysis results of fungal diversity was also discussed. The results showed that the mildew dark tea fungus under high humidity conditions were mainly classified into 2 phylum (Ascomycota, Basidiomycota) and 6 genera (Aspergillus, Galactomyces, Ogataea, Debaryomyces, Pichia and Cryptococcus), with the highest abundance value of Aspergillus (>98%). The different OTU thresholds resulted in the difference in the taxonomic status, diversity index and community structure in the same sample. The Shannon diversity index of OTU under the 0.97 was 0.15-0.43, and the Shannon diversity index under 0.99 was 0.28-0.58. Under high moisture conditions aspergillus was the major fungi causing dark tea mildew in storage.

Key words

fungi community diversity / mildew / raw dark tea

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XU Wei, JIANG Yihe, WU Dan, ZHAO Renliang, ZHU Qi. RNA Sequencing Analysis of Fungi Community Diversity in Mildew Raw Dark Tea[J]. Journal of Tea Science. 2017, 37(5): 483-492

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