Cloning and Expression Analysis of CsBON3 from Copine Gene Family in Tea Plant (Camellia sinensis)

YAO Lina; HAO Xinyuan; WANG Lu; LI Nana; ZENG Jianming; YANG Yajun; WANG Xinchao

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Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (6) : 565-574.

Cloning and Expression Analysis of CsBON3 from Copine Gene Family in Tea Plant (Camellia sinensis)

YAO Lina, HAO Xinyuan, WANG Lu, LI Nana, ZENG Jianming, YANG Yajun^*, WANG Xinchao^*

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Abstract

Copine protein, a kind of Ca²⁺-dependent protein or phospholipid-binding protein containing two C2 (N-terminal) and one vWA (C-terminal) conserved domain, plays an important role in intracellular signal transduction. Based on the sequence identity analysis, an EST sequence showing high homology to Copine family genes was selected from our previous tea plant transcriptome database. The sequence was verified to contain 1β746βbp complete ORF, encoding 581 amino acids. Homologous alignment showed that the gene had the highest similarity with Arabidopsis AtBON3 (65%) and was named as CsBON3 (GenBank accession number: KY435900). Bioinformatic analysis showed that the molecular weight of CsBON3 protein was 63.66βkD and the theoretical isoelectric point was 5.48. It possesses the conserved domain unique to Copine family protein, which is a hydrophilic protein, no signal peptide site, non-secretory protein and no transmembrane domain. Expression analysis showed that CsBON3 had the highest expression levels in tea flowers and roots, and the lowest in stems and mature leaves. It was significantly up-regulated in tea plants treated with low temperature (4℃) for one day, and its expression was suppressed at dormant stage and up-regulated at growth stage. It was also up-regulated rapidly in the leaves with inoculation of Colletotrichum fructicola. The results indicated that the CsBON3 plays important roles in tea plant growth in response to low temperature and disease resistance.

Key words

BON gene / Copine family / dormancy / expression analysis / stress response / tea plant

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YAO Lina, HAO Xinyuan, WANG Lu, LI Nana, ZENG Jianming, YANG Yajun, WANG Xinchao. Cloning and Expression Analysis of CsBON3 from Copine Gene Family in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science. 2017, 37(6): 565-574

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