Anti-obesity Efficacy of Shoumei White Tea on High-fat Diet Mice and Its Safety Evaluation

ZHENG Li; HOU Caiyun; REN Fazheng

PDF(1198 KB)

Journal of Tea Science ›› 2017, Vol. 37 ›› Issue (6) : 586-596.

Anti-obesity Efficacy of Shoumei White Tea on High-fat Diet Mice and Its Safety Evaluation

ZHENG Li¹, HOU Caiyun^1,2,*, REN Fazheng²

Author information +

History +

Abstract

White tea extracts (WTEs) had been prepared from Shoumei white tea stored for 1, 3 and 7 years, respectively. Male mice (C57BL/6J) were fed with high-fat diet and supplemented with WTE to establish the anti-obesity efficacy model. Body weight gain, food intake, the pathobiology changes of the liver and adipose tissues, serum lipids profiles and differential expression profiles of lipid metabolism-related genes of liver in mice were investigated. The results showed that WTEs inhibited adipocyte differentiation and lipid excessive accumulation in adipose tissues, inhibited fatty acid synthesis and lipid excessive accumulation in liver by ameliorating the expression levels of lipid metabolism-related genes via down-regulation of the mRNA expression level of SREBP-1c, FAS and ACC1, thus depressing the increase of body weight and adipose tissues induced by high-fat diet. The preventing obesity efficacy of Shoumei white tea in high dosage was reduced with the increase of the storage years..

Key words

anti-obesity / safety / white tea

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

ZHENG Li, HOU Caiyun, REN Fazheng. Anti-obesity Efficacy of Shoumei White Tea on High-fat Diet Mice and Its Safety Evaluation[J]. Journal of Tea Science. 2017, 37(6): 586-596

References

[1] 中国国家标准化管理委员会. GB/T 30766—2014 茶叶分类[S]. 北京: 中国标准出版社, 2014: 2.
[2] 叶乃兴. 白茶、白叶茶与白毛茶[J]. 福建茶叶, 2010, 10: 44-46.
[3] 刘国根, 罗泽民, 邱冠周, 等. 茶叶中自由基的研究[J]. 湖南农业大学学报: 自然科学版, 1999, 25(4): 290-292.
[4] 中华人民共和国国家质量监督检验检疫总局. GB/T 8313-2008. 中华人民共和国国家标准-茶叶中茶多酚和儿茶素含量的检测方法[S]. 北京: 中国标准出版社, 2008-05-04.
[5] 中华人民共和国国家质量监督检验检疫总局. GB/T 8312-2013. 中华人民共和国国家标准-茶-咖啡碱测定[S]. 北京: 中国标准出版社, 2013-12-31.
[6] 马陶陶, 张群林, 李俊, 等. 三氯化铝比色法测定中药总黄酮方法的探讨[J]. 时珍国医国药, 2008, 19(1): 54-56.
[7] 王蝶. 茶叶对肥胖大鼠的减肥作用及机制研究[D]. 长沙: 湖南农业大学, 2012: 10-11.
[8] 黄继汉, 黄晓晖, 陈志扬, 等. 药理试验中动物间和动物与人体间的等效剂量换算[J]. 中国临床药理学与治疗学, 2004, 9(9): 1069-1072.
[9] 贺争鸣. 实验动物管理与使用指南[M]. 北京: 科学出版社, 2016: 142-150.
[10] 章金涛. 医学实验动物学[M]. 郑州: 郑州大学出版社, 2014: 385-387.
[11] Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-timequantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001, 25(4): 402-408.
[12] Kautzky W A, Lemmens G R.Obesity and diabetes[M]//Regitz Zagrosek V. Sex and gender differences in pharmacology. Heidelberg: Springer, 2013: 307-340.
[13] Aherne S A, O'Brien N M. Dietary flavonols: chemistry, food content, and metabolism[J]. Nutrition, 2002, 18(1): 75-81.
[14] Gondoin A, Grussu D, Stewart D, et al.White and green tea polyphenols inhibit pancreatic lipase in vitro[J]. Food Research International, 2010, 43(5): 1537-1544.
[15] Martins F, Noso TM, Porto VB, et al.Maté tea inhibits in vitro pancreatic lipase activity and has hypolpidemic effect on high-fat diet-induced obese mice[J]. Obesity, 2009, 18(1): 42-47.
[16] Westerterp-Plantenga M S, Mattes R. Green tea catechins, caffeine and body-weight regulation[J]. Physiology & Behavior, 2010, 100(1): 42-46.
[17] 王丽娟, 李晓蓉, 李宇航, 等. 高脂饲料对C57BL/6J小鼠血脂与转氨酶的动态影响[J]. 2007, 17(10): 572-576.
[18] Nishiumi S, Bessyo H, Kubo M, et al.Green and black tea suppress hyperglycemia andInsulin resistance by retaining the expression of glucose transporter 4 in muscle of high-fat diet-fed C57BL/6J mice[J]. Journal of Agricultural & Food Chemistry, 2010, 58(24): 12916-12923.
[19] Byun J K, Yoon B Y, Jhun J Y, et al.Epigallocatechin- 3-gallate ameliorates both obesity and auto inflammatory arthritis aggravated by obesity by altering the balance among CD⁴+ T-cellsubsets[J]. Immunology Letters, 2014, 157(12): 51-58.
[20] Mousumi B, Joshua D. Lambert J, et al.The major green tea polyphenol, (-)-epigallocatechin-3-gallate, inhibits obesity, metabolic syndrome, and fatty liver disease in high-fat-fed mice[J]. Journal of Nutrition, 2008, 138(9): 1677-1683.
[21] Chen YK, Cheung C, Reuhl KR, et al.Effects of green tea polyphenol (-)-epigallocatechin-3-gallate on a newly developed high-fat/western-style Ddiet-induced obesity and metabolic syndrome in mice[J]. Journal of Agricultural & Food Chemistry, 2011, 59(21): 11862-11871.
[22] Murase, TNagasawa, ASuzuki, et al. Beneficial effects of tea catechins on diet-induced obes-ity: stimulation of lipid catabolism in the liver[J]. International Journal of Obesity, 2002, 26(11): 1459-1464.
[23] 陈国良, 刘立伟, 谢爽, 等. 高密度脂蛋白胆固醇代谢及其对冠心病影响的研究进展[J]. 心血管病学进展, 2010, 31(3): 360-363.
[24] Rizzo M, Otvos J, Nikolic D, et al.Subfractions and subpopulations of HDL: an update[J]. Current Medicinal Chemistry, 2014, 21(25): 2881-2891.
[25] Kozarsky K F, Donahee M H, Rigotti A, et al.Overexpression of the HDL receptor SR-BIalters plasma HDL and bile cholesterol levels[J]. Nature, 1997, 387(6631): 414-417.
[26] Rigotti A, Trigatti B L, Penman M, et al.A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism[J]. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(23): 12610-12615.
[27] 杨永青. 脂肪细胞分化及其调控机制[M]. 北京: 中国农业科学技术出版社, 2014: 12-14.