The Functional Identification of Two Alternative Splicing Transcripts of CsNES

ZHOU Hanchen; LEI Pandeng

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (6) : 753-760.

Research Paper

The Functional Identification of Two Alternative Splicing Transcripts of CsNES

ZHOU Hanchen, LEI Pandeng^*

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Abstract

CsNES is a key enzyme for the formation of (E)-nerolidol in tea plants. According to the analysis of full-length transcriptome and genome data, it is shown that CsNES has two shorter alternative splicing transcripts besides the full-length transcript. The rapid amplification of cDNA ends (RACE) result indicates that the CsNES undergoes 5' alternative splicing in the second and third exons, respectively, which were named as CsNES-2 and CsNES-3. The recombinant proteins of CsNES-2 and CsNES-3 possess strong activities to catalyze the FPP into (E)-nerolidol and weak activities to hydrolyze FPP into (Z)-nerolidol. The gene expression analysis shows that CsNES-2 and CsNES-3 had higher expression levels in tea flower compared to those in tea leaves. Their expressions in young leaves were higher than those in mature leaves. Furthermore, the expression levels of CsNES-2 and CsNES-3 were induced by the MeJA treatment. This study identified two alternative splicing transcripts of the CsNES gene and gave insights into the regulation of gene alternative splicing in terpenoids metabolism in Camellia sinensis.

Key words

Camellia sinensis / alternative splicing / expression analysis / function analysis / nerolidol synthase

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ZHOU Hanchen, LEI Pandeng. The Functional Identification of Two Alternative Splicing Transcripts of CsNES[J]. Journal of Tea Science. 2021, 41(6): 753-760

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