Purine Alkaloid Evaluation and Excellent Resources Screening of Fujian Wild Tea

CHEN Xiaomin; ZHAO Feng; WANG Shuyan; SHAO Shuxian; WU Wenxi; LIN Qin; WANG Pengjie; YE Naixing

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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (1) : 18-28.

Research Paper

Purine Alkaloid Evaluation and Excellent Resources Screening of Fujian Wild Tea

CHEN Xiaomin¹, ZHAO Feng^2,*, WANG Shuyan¹, SHAO Shuxian¹, WU Wenxi³, LIN Qin⁴, WANG Pengjie¹, YE Naixing^1,*

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Abstract

A total of forty-three wild tea resources in Fujian Province were applied in the research and the contents of purine alkaloids were determined by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The descriptive statistical analysis and cluster analysis were carried out to systematically analyze and explore the composition characteristics of purine alkaloids in Fujian wild tea resources. The results show that large variations in the purine alkaloids were identified in tea resources, which could be divided into four specific groups: group Ⅰ with similar contents of purine alkaloids as those in normal tea cultivars, group Ⅱ with higher purine alkaloids especially high caffeine content and low theophylline and theobromine, group Ⅲ with higher purine alkaloids especially high bitter theophylline and theophylline contents, group Ⅳ with high purine alkaloids especially bitter theophylline. After comprehensive evaluation, 14 tea resources with specific purine alkaloid composition were identified, including five germplasm resources with high purine alkaloids (>60 mg·g^-1), seven germplasm resources with high caffeine (>50 mg·g^-1) and seven germplasm resources with high bitter theophylline (>30 mg·g^-1). Through this study, it provided more information and a scientific basis for the protection, innovation, breeding, production and utilization of tea germplasm resources.

Key words

germplasm resources / liquid chromatography-mass spectrometry / purine alkaloid / tea plant / theacrine

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CHEN Xiaomin, ZHAO Feng, WANG Shuyan, SHAO Shuxian, WU Wenxi, LIN Qin, WANG Pengjie, YE Naixing. Purine Alkaloid Evaluation and Excellent Resources Screening of Fujian Wild Tea[J]. Journal of Tea Science. 2022, 42(1): 18-28

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