福建野生茶树资源嘌呤生物碱构成评价及特异资源筛选

Abstract

Abstract: A total of forty-three wild tea resources in Fujian Province were applied in the research and the contents of purine alkaloids were determined by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The descriptive statistical analysis and cluster analysis were carried out to systematically analyze and explore the composition characteristics of purine alkaloids in Fujian wild tea resources. The results show that large variations in the purine alkaloids were identified in tea resources, which could be divided into four specific groups: group Ⅰ with similar contents of purine alkaloids as those in normal tea cultivars, group Ⅱ with higher purine alkaloids especially high caffeine content and low theophylline and theobromine, group Ⅲ with higher purine alkaloids especially high bitter theophylline and theophylline contents, group Ⅳ with high purine alkaloids especially bitter theophylline. After comprehensive evaluation, 14 tea resources with specific purine alkaloid composition were identified, including five germplasm resources with high purine alkaloids (>60 mg·g^-1), seven germplasm resources with high caffeine (>50 mg·g^-1) and seven germplasm resources with high bitter theophylline (>30 mg·g^-1). Through this study, it provided more information and a scientific basis for the protection, innovation, breeding, production and utilization of tea germplasm resources.

Key words: tea plant, germplasm resources, purine alkaloid, theacrine, liquid chromatography-mass spectrometry

CLC Number:

CHEN Xiaomin, ZHAO Feng, WANG Shuyan, SHAO Shuxian, WU Wenxi, LIN Qin, WANG Pengjie, YE Naixing. Purine Alkaloid Evaluation and Excellent Resources Screening of Fujian Wild Tea[J]. Journal of Tea Science, 2022, 42(1): 18-28.

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Purine Alkaloid Evaluation and Excellent Resources Screening of Fujian Wild Tea

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