Determination of Anthocyanin-like Substances in Tea by High Performance Liquid Chromatography

YE Shuixin; WANG Weiwei; ZHANG Mengxue; JIANG Heyuan; ZHANG Haihua

doi:10.13305/j.cnki.jts.2025.03.009

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Journal of Tea Science ›› 2025, Vol. 45 ›› Issue (3) : 454-464. DOI: 10.13305/j.cnki.jts.2025.03.009

Research Paper

Determination of Anthocyanin-like Substances in Tea by High Performance Liquid Chromatography

YE Shuixin^1,2, WANG Weiwei^2,3, ZHANG Mengxue^2,4, JIANG Heyuan^2,3,*, ZHANG Haihua^1,*

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Abstract

In order to establish a high performance liquid chromatographic (HPLC) method for the analysis of anthocyanin-like substances in tea, six typical anthocyanins (delphinidin, cyanidin, petunidin chloride, pelargonidin, paeonidin and malvidin) were used as the targets, and Cosmosil 5C₁₈-AR-Ⅱ chromatographic column (250 mm×4.6 mm, 5 μm) was used with the mobile phases of 0.5% formic acid in water and 100% acetonitrile as mobile phases A and B, respectively. The column temperature was 35 ℃, the flow rate was 0.8 mL·min^-1, the detection wavelength was 525 nm, and the injection volume was 20 μL. Under the analytical conditions, the anthocyanin components were well separated. In a certain range, the linear relationship was significant, and the correlation coefficient square (R²) was above 0.999 0. The relative standard deviations (RSD) of the repeatability, precision and stability tests were in the range of 1.20%-1.40%, 1.15%-2.12% and 1.35%-3.38%, respectively. The results of the spiked recovery test show that the average recoveries of the anthocyanin components were in the range of 86.08%-110.34% with an RSD between 0.24% and 6.13%. The method has good reproducibility, precision and stability, and can detect the contents of anthocyanin-like substances in tea.

Key words

anthocyanin / high performance liquid chromatography (HPLC) / tea

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YE Shuixin, WANG Weiwei, ZHANG Mengxue, JIANG Heyuan, ZHANG Haihua. Determination of Anthocyanin-like Substances in Tea by High Performance Liquid Chromatography[J]. Journal of Tea Science. 2025, 45(3): 454-464 https://doi.org/10.13305/j.cnki.jts.2025.03.009

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