高效液相色谱法检测茶叶中花青素类物质含量

Abstract

Abstract: In order to establish a high performance liquid chromatographic (HPLC) method for the analysis of anthocyanin-like substances in tea, six typical anthocyanins (delphinidin, cyanidin, petunidin chloride, pelargonidin, paeonidin and malvidin) were used as the targets, and Cosmosil 5C₁₈-AR-Ⅱ chromatographic column (250 mm×4.6 mm, 5 μm) was used with the mobile phases of 0.5% formic acid in water and 100% acetonitrile as mobile phases A and B, respectively. The column temperature was 35 ℃, the flow rate was 0.8 mL·min^-1, the detection wavelength was 525 nm, and the injection volume was 20 μL. Under the analytical conditions, the anthocyanin components were well separated. In a certain range, the linear relationship was significant, and the correlation coefficient square (R²) was above 0.999 0. The relative standard deviations (RSD) of the repeatability, precision and stability tests were in the range of 1.20%-1.40%, 1.15%-2.12% and 1.35%-3.38%, respectively. The results of the spiked recovery test show that the average recoveries of the anthocyanin components were in the range of 86.08%-110.34% with an RSD between 0.24% and 6.13%. The method has good reproducibility, precision and stability, and can detect the contents of anthocyanin-like substances in tea.

Key words: anthocyanin, high performance liquid chromatography (HPLC), tea

CLC Number:

YE Shuixin, WANG Weiwei, ZHANG Mengxue, JIANG Heyuan, ZHANG Haihua. Determination of Anthocyanin-like Substances in Tea by High Performance Liquid Chromatography[J]. Journal of Tea Science, 2025, 45(3): 454-464.

References

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Determination of Anthocyanin-like Substances in Tea by High Performance Liquid Chromatography

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